Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds

Introduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parame...

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Main Authors: Carolina LOZAN-TIRSU, Elena ZARICIUC
Format: Article
Language:English
Published: Asociatia de Biosiguranta si Biosecuritate 2021-06-01
Series:One Health & Risk Management
Subjects:
ct
sod
Online Access:https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/140/98
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spelling doaj-f82964c2d0a949aba87c3a8589fadeee2021-06-18T08:40:33ZengAsociatia de Biosiguranta si BiosecuritateOne Health & Risk Management2887-34582587-34662021-06-0123556010.38045/ohrm.2021.3.09Biochemical composition changes of gram-negative microorganisms under the action of new chemical compoundsCarolina LOZAN-TIRSU0https://orcid.org/0000-0003-3831-2758Elena ZARICIUC1https://orcid.org/0000-0002-6966-218XDiscipline of microbiology and immunology, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of MoldovaDiscipline of microbiology and immunology, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of MoldovaIntroduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parameters of the antioxidant-enzyme activities within the reference cultures under the action of selected new chemical compounds. Material and methods. Cu (II) coordination compounds, Co (II) and Zn (II) were included as “in vitro” study material, as well as aromatic propenones synthesized at the Department of Inorganic Chemistry, at State University of Moldova. The antimicrobial effects were tested on three gram-negative reference strains. The enzymatic activity of SOD and the CT activity was determined to assess the pathogen-induced changes in the antioxidant status under the action of newly tested compounds. Results. The new chemical compounds showed a significant decrease in the antioxidant enzymatic activities of SOD and CT, induced in all reference strains used, which indicates an intense oxidative stress generated by the tested compounds. Conclusions. The specific activity of new chemical compounds on primary antioxidant enzymes, which represent pathogenicity factors of infectious agents, allows us to assume the benefits of in vivo effect of new native substances, as well as to recommend the selected compounds for further biomedical tests.https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/140/98antimicrobial activityantioxidant-enzyme activitychemical compoundsctsod
collection DOAJ
language English
format Article
sources DOAJ
author Carolina LOZAN-TIRSU
Elena ZARICIUC
spellingShingle Carolina LOZAN-TIRSU
Elena ZARICIUC
Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
One Health & Risk Management
antimicrobial activity
antioxidant-enzyme activity
chemical compounds
ct
sod
author_facet Carolina LOZAN-TIRSU
Elena ZARICIUC
author_sort Carolina LOZAN-TIRSU
title Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_short Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_full Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_fullStr Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_full_unstemmed Biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
title_sort biochemical composition changes of gram-negative microorganisms under the action of new chemical compounds
publisher Asociatia de Biosiguranta si Biosecuritate
series One Health & Risk Management
issn 2887-3458
2587-3466
publishDate 2021-06-01
description Introduction. It is known that the enzymes catalase (CT) and superoxide dismutase (SOD) are actively involved in protecting cells of microbial pathogens against the biochemical factors produced by macrophages in vivo, in order to stop infection. Therefore, this study was aimed to quantify the parameters of the antioxidant-enzyme activities within the reference cultures under the action of selected new chemical compounds. Material and methods. Cu (II) coordination compounds, Co (II) and Zn (II) were included as “in vitro” study material, as well as aromatic propenones synthesized at the Department of Inorganic Chemistry, at State University of Moldova. The antimicrobial effects were tested on three gram-negative reference strains. The enzymatic activity of SOD and the CT activity was determined to assess the pathogen-induced changes in the antioxidant status under the action of newly tested compounds. Results. The new chemical compounds showed a significant decrease in the antioxidant enzymatic activities of SOD and CT, induced in all reference strains used, which indicates an intense oxidative stress generated by the tested compounds. Conclusions. The specific activity of new chemical compounds on primary antioxidant enzymes, which represent pathogenicity factors of infectious agents, allows us to assume the benefits of in vivo effect of new native substances, as well as to recommend the selected compounds for further biomedical tests.
topic antimicrobial activity
antioxidant-enzyme activity
chemical compounds
ct
sod
url https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/140/98
work_keys_str_mv AT carolinalozantirsu biochemicalcompositionchangesofgramnegativemicroorganismsundertheactionofnewchemicalcompounds
AT elenazariciuc biochemicalcompositionchangesofgramnegativemicroorganismsundertheactionofnewchemicalcompounds
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