Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction

Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction

Abstract Background The development of an influenza RNA-dependent RNA polymerase (RdRp) inhibitor is required; therefore, a method for evaluating the activity of influenza RdRp needs to be developed. The current method uses an ultracentrifuge to separate viral particles and quantifies RdRp activity...

Full description

Bibliographic Details
Main Authors: Yuka Horio, Mototada Shichiri, Yuji Isegawa
Format: Article
Language:English
Published: BMC 2021-08-01
Series:Virology Journal
Subjects:
Influenza virus
RNA-dependent RNA polymerase activity
Real-time reverse transcription polymerase chain reaction
Ribavirin
Online Access:https://doi.org/10.1186/s12985-021-01644-7

Similar Items

Similar Items