Determination of long-chain base in glycosphingolipids with fluorescamine

A method is described for determining the longchain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.

Bibliographic Details
Main Authors:	Alemka Kisic, Maurice M. Rapport
Format:	Article
Language:	English
Published:	Elsevier 1974-03-01
Series:	Journal of Lipid Research
Subjects:	fluorescence sphingosine galactosyl ceramide cytolipin H cytolipin K cytolipin R
Online Access:	http://www.sciencedirect.com/science/article/pii/S002222752036822X

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spelling	doaj-f7c8d40ff46e4248b42a7ee96b1569dd2021-04-24T05:49:17ZengElsevierJournal of Lipid Research0022-22751974-03-01152179180Determination of long-chain base in glycosphingolipids with fluorescamineAlemka Kisic0Maurice M. Rapport1Division of Neuroscience, New York State Psychiatric Institute, and Department of Biochemistry, Columbia University College of Physicians and Surgeons, New York 10032Division of Neuroscience, New York State Psychiatric Institute, and Department of Biochemistry, Columbia University College of Physicians and Surgeons, New York 10032A method is described for determining the longchain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.http://www.sciencedirect.com/science/article/pii/S002222752036822Xfluorescencesphingosinegalactosyl ceramidecytolipin Hcytolipin Kcytolipin R
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Alemka Kisic Maurice M. Rapport
spellingShingle	Alemka Kisic Maurice M. Rapport Determination of long-chain base in glycosphingolipids with fluorescamine Journal of Lipid Research fluorescence sphingosine galactosyl ceramide cytolipin H cytolipin K cytolipin R
author_facet	Alemka Kisic Maurice M. Rapport
author_sort	Alemka Kisic
title	Determination of long-chain base in glycosphingolipids with fluorescamine
title_short	Determination of long-chain base in glycosphingolipids with fluorescamine
title_full	Determination of long-chain base in glycosphingolipids with fluorescamine
title_fullStr	Determination of long-chain base in glycosphingolipids with fluorescamine
title_full_unstemmed	Determination of long-chain base in glycosphingolipids with fluorescamine
title_sort	determination of long-chain base in glycosphingolipids with fluorescamine
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1974-03-01
description	A method is described for determining the longchain base content of glycosphingolipids after acid hydrolysis, using the new reagent fluorescamine. The reaction is sensitive and can be used to characterize or measure glycosphingolipids in quantities routinely separated by thin-layer chromatography.
topic	fluorescence sphingosine galactosyl ceramide cytolipin H cytolipin K cytolipin R
url	http://www.sciencedirect.com/science/article/pii/S002222752036822X
work_keys_str_mv	AT alemkakisic determinationoflongchainbaseinglycosphingolipidswithfluorescamine AT mauricemrapport determinationoflongchainbaseinglycosphingolipidswithfluorescamine
_version_	1721511881728851968

Determination of long-chain base in glycosphingolipids with fluorescamine

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