Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation

The dynamics of skin-draining cells following infection or vaccination provide important insight into the initiation of immune responses. In this study, the local recruitment and activation of immune cells in draining lymph nodes (LNs) was studied in calves in an adjuvant-induced inflammation. A tra...

Full description

Bibliographic Details
Main Authors: Hege Lund, Preben Boysen, Caroline Piercey Åkesson, Anna Monika Lewandowska-Sabat, Anne Storset
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-08-01
Series:Frontiers in Immunology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00322/full
id doaj-f7bc0bd9aa994842b9765ca09da96dbd
record_format Article
spelling doaj-f7bc0bd9aa994842b9765ca09da96dbd2020-11-25T01:08:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242016-08-01710.3389/fimmu.2016.00322218196Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammationHege Lund0Preben Boysen1Caroline Piercey Åkesson2Anna Monika Lewandowska-Sabat3Anne Storset4Norwegian University of Life SciencesNorwegian University of Life SciencesNorwegian University of Life SciencesNorwegian University of Life SciencesNorwegian University of Life SciencesThe dynamics of skin-draining cells following infection or vaccination provide important insight into the initiation of immune responses. In this study, the local recruitment and activation of immune cells in draining lymph nodes (LNs) was studied in calves in an adjuvant-induced inflammation. A transient but remarkably strong recruitment of monocytes was demonstrated after onset of inflammation, constituting up to 41 % of live cells in the draining LNs after 24 h. Numerous CD14+ cells were visualized in subcutaneous tissues and draining LNs, and the majority of these cells did not express dendritic cell-associated markers CD205 and CD11c. In the LNs, recruited cells were predominately of a CD14++ and CD16+ phenotype, consistent with an intermediate monocyte subset characterized to possess a high inflammatory potential. Moreover, monocytes from the draining lymph node showed a high expression of genes coding for pro-inflammatory cytokines, including IL-1β, IL-6, TNFa and TGFβ. Shortly after their appearance in the LN cortical areas, the monocytes had moved into the medulla followed by an increase in peripheral blood. In conclusion, this study provides novel information on in vivo monocyte recruitment and migration after onset of inflammation.http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00322/fullInflammationMonocytesMigrationPro-inflammatory cytokinesLymph Node
collection DOAJ
language English
format Article
sources DOAJ
author Hege Lund
Preben Boysen
Caroline Piercey Åkesson
Anna Monika Lewandowska-Sabat
Anne Storset
spellingShingle Hege Lund
Preben Boysen
Caroline Piercey Åkesson
Anna Monika Lewandowska-Sabat
Anne Storset
Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation
Frontiers in Immunology
Inflammation
Monocytes
Migration
Pro-inflammatory cytokines
Lymph Node
author_facet Hege Lund
Preben Boysen
Caroline Piercey Åkesson
Anna Monika Lewandowska-Sabat
Anne Storset
author_sort Hege Lund
title Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation
title_short Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation
title_full Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation
title_fullStr Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation
title_full_unstemmed Transient migration of large numbers of CD14++ CD16+ monocytes to the draining lymph node after onset of inflammation
title_sort transient migration of large numbers of cd14++ cd16+ monocytes to the draining lymph node after onset of inflammation
publisher Frontiers Media S.A.
series Frontiers in Immunology
issn 1664-3224
publishDate 2016-08-01
description The dynamics of skin-draining cells following infection or vaccination provide important insight into the initiation of immune responses. In this study, the local recruitment and activation of immune cells in draining lymph nodes (LNs) was studied in calves in an adjuvant-induced inflammation. A transient but remarkably strong recruitment of monocytes was demonstrated after onset of inflammation, constituting up to 41 % of live cells in the draining LNs after 24 h. Numerous CD14+ cells were visualized in subcutaneous tissues and draining LNs, and the majority of these cells did not express dendritic cell-associated markers CD205 and CD11c. In the LNs, recruited cells were predominately of a CD14++ and CD16+ phenotype, consistent with an intermediate monocyte subset characterized to possess a high inflammatory potential. Moreover, monocytes from the draining lymph node showed a high expression of genes coding for pro-inflammatory cytokines, including IL-1β, IL-6, TNFa and TGFβ. Shortly after their appearance in the LN cortical areas, the monocytes had moved into the medulla followed by an increase in peripheral blood. In conclusion, this study provides novel information on in vivo monocyte recruitment and migration after onset of inflammation.
topic Inflammation
Monocytes
Migration
Pro-inflammatory cytokines
Lymph Node
url http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00322/full
work_keys_str_mv AT hegelund transientmigrationoflargenumbersofcd14cd16monocytestothedraininglymphnodeafteronsetofinflammation
AT prebenboysen transientmigrationoflargenumbersofcd14cd16monocytestothedraininglymphnodeafteronsetofinflammation
AT carolinepierceyakesson transientmigrationoflargenumbersofcd14cd16monocytestothedraininglymphnodeafteronsetofinflammation
AT annamonikalewandowskasabat transientmigrationoflargenumbersofcd14cd16monocytestothedraininglymphnodeafteronsetofinflammation
AT annestorset transientmigrationoflargenumbersofcd14cd16monocytestothedraininglymphnodeafteronsetofinflammation
_version_ 1725183837840867328