PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens

Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.

Bibliographic Details
Main Authors: Iain J. MacLeod, Christopher F. Rowley, M. Essex
Format: Article
Language:English
Published: Nature Publishing Group 2021-02-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-021-01751-9
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spelling doaj-f79a51c5dc574d0197305537b3b9d5a52021-02-21T12:27:34ZengNature Publishing GroupCommunications Biology2399-36422021-02-014111310.1038/s42003-021-01751-9PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogensIain J. MacLeod0Christopher F. Rowley1M. Essex2Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public HealthDepartment of Immunology and Infectious Diseases, Harvard TH Chan School of Public HealthDepartment of Immunology and Infectious Diseases, Harvard TH Chan School of Public HealthIain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.https://doi.org/10.1038/s42003-021-01751-9
collection DOAJ
language English
format Article
sources DOAJ
author Iain J. MacLeod
Christopher F. Rowley
M. Essex
spellingShingle Iain J. MacLeod
Christopher F. Rowley
M. Essex
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
Communications Biology
author_facet Iain J. MacLeod
Christopher F. Rowley
M. Essex
author_sort Iain J. MacLeod
title PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_short PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_full PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_fullStr PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_full_unstemmed PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
title_sort pandaa intentionally violates conventional qpcr design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
publisher Nature Publishing Group
series Communications Biology
issn 2399-3642
publishDate 2021-02-01
description Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.
url https://doi.org/10.1038/s42003-021-01751-9
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AT christopherfrowley pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens
AT messex pandaaintentionallyviolatesconventionalqpcrdesigntoenabledurablemismatchagnosticdetectionofhighlypolymorphicpathogens
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