PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
Iain MacLeod et al. develop the Pan-Degenerate Amplification and Adaptation (PANDAA) point mutation assay to detect HIV drug resistance mutations. This method can be applied to any pathogen where target-proximal genetic variability hinders diagnostic development.
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2021-02-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-01751-9 |