| Summary: | The objective of this work is the validation and implementation of an analytical method for the determination of arsenic chemical species in rice grain samples using High-performance liquid chromatography coupled to a hydride generator with atomic fluorescence detector (HPLC-HG-AFS). The extraction protocol developed was based on HNO3 0.28 M (90 °C, 2 h), microwave-assisted. The results showed recovery percentages of arsenite (As (III)) (99–101%), arsenate (As (V)) (91–96%), dimethylarsinic acid (DMA) (92–102%) and monomethylarsonic acid (MMA) (94–97%). The precision of the method presented coefficients of variation lower than 7% and 8% for repeatability and reproducibility respectively. The detection limits were 2.5, 3.75, 7.5 and 4.0 µg kg−1 for As (III), As (V), DMA and MMA respectively. The proposed methodology is reliable for the quantification of As species, because they are conserved during the extraction. • The extraction protocol developed was based on Microwave-assisted acid extraction. • This methodology offers good sensitivity, precision, accuracy, detection and quantification limits. • It was successfully applied to determination of arsenic chemical species in rice grains.
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