Purification and characterization of a DNA-binding recombinant PREP1:PBX1 complex.

Purification and characterization of a DNA-binding recombinant PREP1:PBX1 complex.

Human PREP1 and PBX1 are homeodomain transcriptional factors, whose biochemical and structural characterization has not yet been fully described. Expression of full-length recombinant PREP1 (47.6 kDa) and PBX1 (46.6 kDa) in E. coli is difficult because of poor yield, high instability and insufficien...

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Bibliographic Details
Main Authors:	Lisa Mathiasen, Chiara Bruckmann, Sebastiano Pasqualato, Francesco Blasi
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2015-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC4391845?pdf=render

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