Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay

This data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (...

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Main Authors:	Min Zhang, Aihong Song, Siqiang Lai, Lisha Qiu, Yunlong Huang, Qiang Chen, Bing Zhu, Dongsheng Xu, Jialin C. Zheng
Format:	Article
Language:	English
Published:	Elsevier 2015-12-01
Series:	Data in Brief
Online Access:	http://www.sciencedirect.com/science/article/pii/S2352340915002425

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spelling	doaj-f77b12f39bc945208633d6ed3d2488152020-11-25T02:29:30ZengElsevierData in Brief2352-34092015-12-015C71271610.1016/j.dib.2015.09.048Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assayMin Zhang0Aihong Song1Siqiang Lai2Lisha Qiu3Yunlong Huang4Qiang Chen5Bing Zhu6Dongsheng Xu7Jialin C. Zheng8Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaCenter for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People’s Hospital affiliated to Tongji University School of Medicine, Shanghai, ChinaThis data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization.http://www.sciencedirect.com/science/article/pii/S2352340915002425
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Min Zhang Aihong Song Siqiang Lai Lisha Qiu Yunlong Huang Qiang Chen Bing Zhu Dongsheng Xu Jialin C. Zheng
spellingShingle	Min Zhang Aihong Song Siqiang Lai Lisha Qiu Yunlong Huang Qiang Chen Bing Zhu Dongsheng Xu Jialin C. Zheng Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay Data in Brief
author_facet	Min Zhang Aihong Song Siqiang Lai Lisha Qiu Yunlong Huang Qiang Chen Bing Zhu Dongsheng Xu Jialin C. Zheng
author_sort	Min Zhang
title	Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_short	Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_full	Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_fullStr	Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_full_unstemmed	Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
title_sort	living cell imaging and rac1-gtp levels of cxcl12-treated migrating neural progenitor cells in stripe assay
publisher	Elsevier
series	Data in Brief
issn	2352-3409
publishDate	2015-12-01
description	This data article contains three figures and three videos related to the research article entitled “Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization” Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization.
url	http://www.sciencedirect.com/science/article/pii/S2352340915002425
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Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay

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