Combining Nanopore and Illumina Sequencing Permits Detailed Analysis of Insertion Mutations and Structural Variations Produced by PEG-Mediated Transformation in <i>Ostreococcus tauri</i>

Combining Nanopore and Illumina Sequencing Permits Detailed Analysis of Insertion Mutations and Structural Variations Produced by PEG-Mediated Transformation in <i>Ostreococcus tauri</i>

<i>Ostreococcus tauri</i> is a simple unicellular green alga representing an ecologically important group of phytoplankton in oceans worldwide. Modern molecular techniques must be developed in order to understand the mechanisms that permit adaptation of microalgae to their environment. W...

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Bibliographic Details
Main Authors: Julie Thomy, Frederic Sanchez, Marta Gut, Fernando Cruz, Tyler Alioto, Gwenael Piganeau, Nigel Grimsley, Sheree Yau
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Cells
Subjects:
Mamiellophyceae
Chlorophyta
polyethylene glycol
copy number
random insertional mutagenesis
unknown sequences
Online Access:https://www.mdpi.com/2073-4409/10/3/664

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