Production of a cellulosic substrate susceptible to enzymatic hydrolysis from prehydrolyzed barley husks

• Main Authors: A.B. MOLDES, J.M. CRUZ, J.M. DOMÍNIQUEZ
• Format: Article
• Language: English
• Published: Scientific Agricultural Society of Finland 2008-12-01
• Series: Agricultural and Food Science
• Online Access: https://journal.fi/afs/article/view/5712

An effective process for the chemical-biotechnological utilization of barley husks is reported. A first treatment with sulfuric acid (prehydrolysis) allowed the solubilization of hemicelluloses to give xylose-containing liquors (suitable to make fermentation media for xylitol production) and a solid phase containing cellulose and lignin. The solid residues from prehydrolysis were treated with NaOH in order to increase their cellulase digestibility. In the alkaline treatments, the effects of temperature (in the range, 50-130ºC), reaction time (10-60 min) and NaOH concentration (3-10 weight percent of solution) on the composition of solid residues were assessed by means of an experimental plan with factorial structure. The cellulose content increased with temperature and NaOH concentration, whereas the duration of treatments was not influential within the range tested. The treated samples showed high susceptibility toward the enzymatic hydrolysis with cellulases, leading to almost quantitative glucose yields under selected operational conditions.