An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.

Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member...

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Main Authors: Yuchuan Zhou, Min Zheng, Qixian Shi, Li Zhang, Wei Zhen, Wenying Chen, Yonglian Zhang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19116669/pdf/?tool=EBI
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spelling doaj-f701d6804e6e4e42ac8e594cee9683b52021-03-03T19:55:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01312e410610.1371/journal.pone.0004106An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.Yuchuan ZhouMin ZhengQixian ShiLi ZhangWei ZhenWenying ChenYonglian ZhangMammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member of the SERPIN (serine proteinase inhibitor) family exclusively expressed in the rat cauda epididymis and up-regulated by androgen, is secreted into the lumen and covers the sperm head. Co-culture of caudal sperms with HongrES1 antibody in vitro resulted in a significant increase in the percentage of capacitated spermatozoa. Furthermore, the percentage of capacitated spermatozoa clearly increased in rats when HongrES1 was down-regulated by RNAi in vivo. Remarkably, knockdown of HongrES1 in vivo led to reduced fertility accompanied with deformed appearance of fetuses and pups. These results identify HongrES1 as a novel and critical molecule in the regulation of sperm capacitation and male fertility.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19116669/pdf/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Yuchuan Zhou
Min Zheng
Qixian Shi
Li Zhang
Wei Zhen
Wenying Chen
Yonglian Zhang
spellingShingle Yuchuan Zhou
Min Zheng
Qixian Shi
Li Zhang
Wei Zhen
Wenying Chen
Yonglian Zhang
An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
PLoS ONE
author_facet Yuchuan Zhou
Min Zheng
Qixian Shi
Li Zhang
Wei Zhen
Wenying Chen
Yonglian Zhang
author_sort Yuchuan Zhou
title An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_short An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_full An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_fullStr An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_full_unstemmed An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_sort epididymis-specific secretory protein hongres1 critically regulates sperm capacitation and male fertility.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2008-01-01
description Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member of the SERPIN (serine proteinase inhibitor) family exclusively expressed in the rat cauda epididymis and up-regulated by androgen, is secreted into the lumen and covers the sperm head. Co-culture of caudal sperms with HongrES1 antibody in vitro resulted in a significant increase in the percentage of capacitated spermatozoa. Furthermore, the percentage of capacitated spermatozoa clearly increased in rats when HongrES1 was down-regulated by RNAi in vivo. Remarkably, knockdown of HongrES1 in vivo led to reduced fertility accompanied with deformed appearance of fetuses and pups. These results identify HongrES1 as a novel and critical molecule in the regulation of sperm capacitation and male fertility.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19116669/pdf/?tool=EBI
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