In Vitro Reconstitution of Yeast Translation System Capable of Synthesizing Long Polypeptide and Recapitulating Programmed Ribosome Stalling

In Vitro Reconstitution of Yeast Translation System Capable of Synthesizing Long Polypeptide and Recapitulating Programmed Ribosome Stalling

The rates of translation elongation or termination in eukaryotes are modulated through cooperative molecular interactions involving mRNA, the ribosome, aminoacyl- and nascent polypeptidyl-tRNAs, and translation factors. To investigate the molecular mechanisms underlying these processes, we developed...

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Bibliographic Details
Main Authors: Riku Nagai, Yichen Xu, Chang Liu, Ayaka Shimabukuro, Nono Takeuchi-Tomita
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Methods and Protocols
Subjects:
CrPV IGR IRES
in vitro translation
translation elongation
translation termination
yeast
Online Access:https://www.mdpi.com/2409-9279/4/3/45

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