A critical evaluation of methods used for S-genotyping: from trees to DNA level

• Main Authors: J. Halász, A. Hegedűs
• Format: Article
• Language: English
• Published: University of Debrecen 2006-04-01
• Series: International Journal of Horticultural Science
• Subjects: F-box; NEpHGE; PCR; Rosaceae; self-incompatibility; S-genotype
• Online Access: https://ojs.lib.unideb.hu/IJHS/article/view/631

Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.