Biobutanol Production from Palm Kernel Cake (PKC) using Clostridium saccharoperbutylacetonicum N1-4 in Batch Culture Fermentation

• Main Authors: Hafiza Shukor, Najeeb Kaid Nasser Al-Shorgani, Peyman Abdeshahian, Aidil Abdul Hamid, Nurina Anuar, Norliza Abd. Rahman, Mohd Hafez Bin Mohd Isa, Mohd Sahaid Kalil
• Format: Article
• Language: English
• Published: North Carolina State University 2014-07-01
• Series: BioResources
• Subjects: Palm kernel cake (PKC); Mannose; Mannan; Biobutanol production; Clostridium saccharoperbutylacetonicum N1-4
• Online Access: http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_3_5325_Shukor_Biobutanol_Palm_Kernel_Cake
• ISSN: 1930-2126; 1930-2126

Palm kernel cake (PKC), a by-product of palm oil industry, contains glucose and mannose as hexose sugars. This study was performed to determine the feasibility of using PKC as a lignocellulosic substrate for biobutanol production by Clostridium saccharoperbutylacetonicum N1-4 in an acetone-butanol-ethanol (ABE) fermentation process. Moreover, the effect of tryptone-yeast extract-acetate (TYA) medium and P2 medium on biobutanol production was evaluated. Experimental results showed that butanol production of 3.05 g/L was obtained using mannose sugar, which was comparable to 3.61 g/L butanol production measured using glucose. Moreover, the maximum production of biobutanol (0.38 g/L) was obtained at a PKC concentration of 30%, indicating the possibility of PKC utilization in butanol production. ABE fermentation of PKC using distilled water, TYA medium, and P2 medium showed that the highest butanol production (0.26 g/L) with ABE production of 0.38 g/L was obtained when ABE fermentation was conducted in P2 medium.