Population genetic structure of <it>Aedes polynesiensis </it>in the Society Islands of French Polynesia: implications for control using a <it>Wolbachia-</it>based autocidal strategy

• Main Authors: Brelsfoard Corey L, Dobson Stephen L
• Format: Article
• Language: English
• Published: BMC 2012-04-01
• Series: Parasites & Vectors
• Subjects: <it>Aedes polynesiensis</it>; Genetic structure; French Polynesia
• Online Access: http://www.parasitesandvectors.com/content/5/1/80

<p>Abstract</p> <p>Background</p> <p><it>Aedes polynesiensis </it>is the primary vector of <it>Wuchereria bancrofti </it>in the South Pacific and an important vector of dengue virus. An improved understanding of the mosquito population genetics is needed for insight into the population dynamics and dispersal, which can aid in understanding the epidemiology of disease transmission and control of the vector. In light of the potential release of a <it>Wolbachia </it>infected strain for vector control, our objectives were to investigate the microgeographical and temporal population genetic structure of <it>A. polynesiensis </it>within the Society Islands of French Polynesia, and to compare the genetic background of a laboratory strain intended for release into its population of origin.</p> <p>Methods</p> <p>A panel of eight microsatellite loci were used to genotype <it>A. polynesiensis </it>samples collected in French Polynesia from 2005-2008 and introgressed <it>A. polynesiensis </it>and <it>Aedes riversi </it>laboratory strains. Examination of genetic differentiation was performed using <it>F</it>-statistics, STRUCTURE, and an AMOVA. BAYESASS was used to estimate direction and rates of mosquito movement.</p> <p>Results</p> <p><it>F</it>_STvalues, AMOVA, and STRUCTURE analyses suggest low levels of intra-island differentiation from multiple collection sites on Tahiti, Raiatea, and Maupiti. Significant pair-wise <it>F</it>_STvalues translate to relatively minor levels of inter-island genetic differentiation between more isolated islands and little differentiation between islands with greater commercial traffic (i.e., Tahiti, Raiatea, and Moorea). STRUCTURE analyses also indicate two population groups across the Society Islands, and the genetic makeup of <it>Wolbachia </it>infected strains intended for release is similar to that of wild-type populations from its island of origin, and unlike that of <it>A. riversi</it>.</p> <p>Conclusions</p> <p>The observed panmictic population on Tahiti, Raiatea, and Moorea is consistent with hypothesized gene flow occurring between islands that have relatively high levels of air and maritime traffic, compared to that of the more isolated Maupiti and Tahaa. Gene flow and potential mosquito movement is discussed in relation to trials of applied autocidal strategies.</p>