Nanoceria Particles Are an Eligible Candidate to Prevent Age-Related Macular Degeneration by Inhibiting Retinal Pigment Epithelium Cell Death and Autophagy Alterations

• Main Authors: Annamaria Tisi, Vincenzo Flati, Simona Delle Monache, Luca Lozzi, Maurizio Passacantando, Rita Maccarone
• Format: Article
• Language: English
• Published: MDPI AG 2020-07-01
• Series: Cells
• Subjects: nanomedicine; nanoceria; retinal pigment epithelium; light damage; autophagy; atrophic AMD
• Online Access: https://www.mdpi.com/2073-4409/9/7/1617

Retinal pigment epithelium (RPE) dysfunction and degeneration underlie the development of age-related macular degeneration (AMD), which is the leading cause of blindness worldwide. In this study, we investigated whether cerium oxide nanoparticles (CeO₂-NPs or nanoceria), which are anti-oxidant agents with auto-regenerative properties, are able to preserve the RPE. On ARPE-19 cells, we found that CeO₂-NPs promoted cell viability against H₂O₂–induced cellular damage. For the in vivo studies, we used a rat model of acute light damage (LD), which mimics many features of AMD. CeO₂-NPs intravitreally injected three days before LD prevented RPE cell death and degeneration and nanoceria labelled with fluorescein were found localized in the cytoplasm of RPE cells. CeO₂-NPs inhibited epithelial-mesenchymal transition of RPE cells and modulated autophagy by the down-regulation of LC3B-II and p62. Moreover, the treatment inhibited nuclear localization of LC3B. Taken together, our study demonstrates that CeO₂-NPs represent an eligible candidate to counteract RPE degeneration and, therefore, a powerful therapy for AMD.