The microRNA gene <i>bta-mir-2313</i> in cattle: an atlas of regulatory elements and an association analysis with growth and carcass traits in the Slovenian Simental cattle breed
<p>MicroRNAs (miRNA) are a class of non-coding RNAs important in posttranscriptional regulation of target genes. Regulation requires complementarity between the target mRNA and the miRNA region responsible for their recognition and binding, also called the seed region. Previous studies hav...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2018-07-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://www.arch-anim-breed.net/61/271/2018/aab-61-271-2018.pdf |
| Summary: | <p>MicroRNAs (miRNA) are a class of non-coding RNAs important in
posttranscriptional regulation of target genes. Regulation requires
complementarity between the target mRNA and the miRNA region responsible for
their recognition and binding, also called the seed region. Previous studies
have proven that expression profiles and genetic variations of miRNA genes
(miR-SNP; SNP – single nucleotide polymorphism) and their target sites
(miR-TS-SNPs) have an impact on phenotypic variation and disease
susceptibility in human, animal models, and livestock. MicroRNA-associated
polymorphisms therefore represent biomarker potential for phenotypic traits
in livestock. Effects of miRNA gene polymorphisms on phenotypic traits have
been studied in several animal species but much less in cattle. The aim of
the present study was therefore to analyze the genetic variability in the
<i>bta-mir-2313</i> gene and test associations with growth and carcass
traits of the Slovenian Simmental cattle breed. Additionally, validated and
predicted genomic information related to the miRNA gene <i>bta-mir-2313</i>
has been obtained and presented as an atlas of miRNA regulatory elements.
Sanger sequencing has been used for biomarker development and genotyping of
145 animals of Slovenian dual-purpose Simmental cattle. Out of nine known
polymorphisms located within pre-miRNA regions, one mature miRNA seed SNP was
polymorphic in the Slovenian Simmental cattle breed. An additional three
polymorphisms were identified within the flanking pri-miRNA regions. There
was no significant effect of polymorphisms on 18 tested fattening and carcass
traits; however, validated polymorphisms could now be tested in association
with other traits in other cattle populations. The microRNA gene
<i>bta-mir-2313</i> warrants further genetic and functional analyses since
it overlaps with a large number of quantitative trait loci (QTL), has over
3100 predicted targets and highly polymorphic mature seed regions, and is
located within protein-coding gene <i>GRAMD1B</i>, previously associated
with production traits in cattle. Mature miRNA seed SNPs present important
genomic loci for functional studies because they could affect the gain/loss
of downstream targets and should be systematically studied in cattle.</p> |
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| ISSN: | 0003-9438 2363-9822 |