Evaluation of bene hull extraction antioxidant activity by superheated water method

• Main Authors: R. Shaddel, M. H. Haddad-Khodaparast, A. Maskooki, A. Sharif, S. Azadmard-Damirchi
• Format: Article
• Language: fas
• Published: Research Institute of Food Science and Technology 2012-06-01
• Series: Pizhūhish va Nuāvarī dar ̒Ulūm va Sanāyi̒-i Ghaz̠āyī
• Subjects: bene hull; melanoides; phenolic compounds; subcritical water
• Online Access: http://journals.rifst.ac.ir/article_66557_70c6fc49414f5a0988fce1449cc2e346.pdf

In the present research, the power of antioxidant activity in Bene Hull subspecies (<em>Mutica)</em> extract was discussed using subcritical water technique, at 110,155 and 200°C temperatures with 45 minutes of constant time and the ratio of  for Bene hull to water. The dependent variables of this study were measured based on Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) and total amount of phenolic compounds (Mg of Gallic Acid/ 100 G of material). The study revealed that at 200°C the Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) were at the maximum; meanwhile total Phenolic content was highest at 155°C. Under this circumstance, the amount of polyphenolic contents, reduction power according to EC₅₀ and DPPH free radical scavenging activity according to EC₅₀ were predicted to be 2096.41 mg Gallic acid / 100 g Bene hull, 0.213 mg /ml and 0.685 mg/ ml, respectively. The results proved that subcritical water extraction method was suitable to obtain biological materials out of Bene hull. Intense extraction yield, abundant solvent, cheap and non-environmental issues are its benefits. <br /><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">In the present research, the power of antioxidant activity in Bene Hull subspecies (<em>Mutica)</em> extract was discussed using subcritical water technique, at 110,155 and 200°C temperatures with 45 minutes of constant time and the ratio of</span> <br /> <br /><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> for </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">Bene hull to water. The dependent variables of this study were measured based on Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) and total amount of phenolic compounds (Mg of Gallic Acid/ 100 G of material).</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> The study revealed that at 200°C the </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) were at the maximum; </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">meanwhile total Phenolic content was highest at 155°C</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">. Under this circumstance, the amount of polyphenolic contents, reduction power according to EC₅₀ and </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">DPPH free radical scavenging activity</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> according to EC₅₀ were predicted to be 2096.41 mg Gallic acid / 100 g </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">Bene</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> hull, 0.213 mg /ml and 0.685 mg/ ml, respect</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">ivel</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">y.</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> The results proved</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> that </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">subcritical water</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> extraction method was</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> suitable to </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">obtain</span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: Calibri; mso-fareast-theme-font: minor-latin; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;"> biological materials out of Bene </span><span style="font-size: 11.0pt; line-height: 115%; font-family: 'Times New Roman','serif'; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">hull. Intense extraction yield, abundant solvent, cheap and non-environmental issues are its benefits</span>