RAPD analysis of genetic diversity and qualitative assessment of hydrolytic activities in a collection of Bacillus sp. isolate

• Main Authors: Berić Tanja, Urdaci Maria C., Stanković S., Knežević-Vukčević Jelena
• Format: Article
• Language: English
• Published: University of Belgrade, University of Novi Sad 2009-01-01
• Series: Archives of Biological Sciences
• Subjects: Bacillus isolates; RAPD; enzyme production
• Online Access: http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640904645B.pdf

Genetic diversity and production of hydrolytic enzymes of 205 Bacillus isolates from different geographical and ecological niches in Serbia were studied. Combining RAPD analysis and 16S DNA sequencing, we determined 13 different groups of RAPD profiles within four (five) species: B. subtilis, B. cereus/B. thuringiensis, B. pumilus, and B. firmus. Screening for production of hydrolytic enzymes showed that there was no correlation of enzyme production with species. Most of the isolates from all habitats produced amylase, protease, lipase, mannanase, and xylanase to some extent at 25ºC and 37ºC. The number of isolates that retained enzyme production ability at 55ºC is considerably lower and they predominantly came from manure.