Genetic relationships among species of the genus Bothrops based on RAPD markers
The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined th...
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Instituto de Tecnologia do Paraná (Tecpar)
2005-05-01
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doaj-f63ab4b844bd410bac8f0d14ab02cc7c2020-11-24T21:06:17ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242005-05-0148335936510.1590/S1516-89132005000300005Genetic relationships among species of the genus Bothrops based on RAPD markersFelipe GrazziotinSergio EcheverrigarayThe genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.<br>As relações genéticas entre 11 espécies de Bothrops (sensus stricto) de ocorrência no Brasil foram determinadas através da comparação dos perfis de fragmentos amplificados aleatoriamente (RAPD). Um total de 239 bandas foram obtidas utilizando 20 sequencias iniciadoras (primers). A maior parte das bandas (88,7%) foram polimórficas. A análise filogenética dos dados permitiu a identificação de três grupos dentro do gênero Bothrops: Grupo 1- B. alternatus, B. neuwiedi, B. cotiara, e B. jararacusu; Grupo II- B. insularis, B. jararaca, e B. erythromelas; e Grupo III- B. moojeni, B. leucurus, e B. atrox. Estes grupos coincidem com aqueles obtidos por outros métodos indicando que os marcadores de RAPD podem ser considerados ferramentas úteis para a avaliação de relações a nível interespecífico.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005BothropsRAPDmolecular markersgenetic relationship |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Felipe Grazziotin Sergio Echeverrigaray |
spellingShingle |
Felipe Grazziotin Sergio Echeverrigaray Genetic relationships among species of the genus Bothrops based on RAPD markers Brazilian Archives of Biology and Technology Bothrops RAPD molecular markers genetic relationship |
author_facet |
Felipe Grazziotin Sergio Echeverrigaray |
author_sort |
Felipe Grazziotin |
title |
Genetic relationships among species of the genus Bothrops based on RAPD markers |
title_short |
Genetic relationships among species of the genus Bothrops based on RAPD markers |
title_full |
Genetic relationships among species of the genus Bothrops based on RAPD markers |
title_fullStr |
Genetic relationships among species of the genus Bothrops based on RAPD markers |
title_full_unstemmed |
Genetic relationships among species of the genus Bothrops based on RAPD markers |
title_sort |
genetic relationships among species of the genus bothrops based on rapd markers |
publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
series |
Brazilian Archives of Biology and Technology |
issn |
1516-8913 1678-4324 |
publishDate |
2005-05-01 |
description |
The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.<br>As relações genéticas entre 11 espécies de Bothrops (sensus stricto) de ocorrência no Brasil foram determinadas através da comparação dos perfis de fragmentos amplificados aleatoriamente (RAPD). Um total de 239 bandas foram obtidas utilizando 20 sequencias iniciadoras (primers). A maior parte das bandas (88,7%) foram polimórficas. A análise filogenética dos dados permitiu a identificação de três grupos dentro do gênero Bothrops: Grupo 1- B. alternatus, B. neuwiedi, B. cotiara, e B. jararacusu; Grupo II- B. insularis, B. jararaca, e B. erythromelas; e Grupo III- B. moojeni, B. leucurus, e B. atrox. Estes grupos coincidem com aqueles obtidos por outros métodos indicando que os marcadores de RAPD podem ser considerados ferramentas úteis para a avaliação de relações a nível interespecífico. |
topic |
Bothrops RAPD molecular markers genetic relationship |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005 |
work_keys_str_mv |
AT felipegrazziotin geneticrelationshipsamongspeciesofthegenusbothropsbasedonrapdmarkers AT sergioecheverrigaray geneticrelationshipsamongspeciesofthegenusbothropsbasedonrapdmarkers |
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1716766068290093056 |