Genetic relationships among species of the genus Bothrops based on RAPD markers

The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined th...

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Main Authors: Felipe Grazziotin, Sergio Echeverrigaray
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2005-05-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005
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spelling doaj-f63ab4b844bd410bac8f0d14ab02cc7c2020-11-24T21:06:17ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242005-05-0148335936510.1590/S1516-89132005000300005Genetic relationships among species of the genus Bothrops based on RAPD markersFelipe GrazziotinSergio EcheverrigarayThe genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.<br>As relações genéticas entre 11 espécies de Bothrops (sensus stricto) de ocorrência no Brasil foram determinadas através da comparação dos perfis de fragmentos amplificados aleatoriamente (RAPD). Um total de 239 bandas foram obtidas utilizando 20 sequencias iniciadoras (primers). A maior parte das bandas (88,7%) foram polimórficas. A análise filogenética dos dados permitiu a identificação de três grupos dentro do gênero Bothrops: Grupo 1- B. alternatus, B. neuwiedi, B. cotiara, e B. jararacusu; Grupo II- B. insularis, B. jararaca, e B. erythromelas; e Grupo III- B. moojeni, B. leucurus, e B. atrox. Estes grupos coincidem com aqueles obtidos por outros métodos indicando que os marcadores de RAPD podem ser considerados ferramentas úteis para a avaliação de relações a nível interespecífico.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005BothropsRAPDmolecular markersgenetic relationship
collection DOAJ
language English
format Article
sources DOAJ
author Felipe Grazziotin
Sergio Echeverrigaray
spellingShingle Felipe Grazziotin
Sergio Echeverrigaray
Genetic relationships among species of the genus Bothrops based on RAPD markers
Brazilian Archives of Biology and Technology
Bothrops
RAPD
molecular markers
genetic relationship
author_facet Felipe Grazziotin
Sergio Echeverrigaray
author_sort Felipe Grazziotin
title Genetic relationships among species of the genus Bothrops based on RAPD markers
title_short Genetic relationships among species of the genus Bothrops based on RAPD markers
title_full Genetic relationships among species of the genus Bothrops based on RAPD markers
title_fullStr Genetic relationships among species of the genus Bothrops based on RAPD markers
title_full_unstemmed Genetic relationships among species of the genus Bothrops based on RAPD markers
title_sort genetic relationships among species of the genus bothrops based on rapd markers
publisher Instituto de Tecnologia do Paraná (Tecpar)
series Brazilian Archives of Biology and Technology
issn 1516-8913
1678-4324
publishDate 2005-05-01
description The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level.<br>As relações genéticas entre 11 espécies de Bothrops (sensus stricto) de ocorrência no Brasil foram determinadas através da comparação dos perfis de fragmentos amplificados aleatoriamente (RAPD). Um total de 239 bandas foram obtidas utilizando 20 sequencias iniciadoras (primers). A maior parte das bandas (88,7%) foram polimórficas. A análise filogenética dos dados permitiu a identificação de três grupos dentro do gênero Bothrops: Grupo 1- B. alternatus, B. neuwiedi, B. cotiara, e B. jararacusu; Grupo II- B. insularis, B. jararaca, e B. erythromelas; e Grupo III- B. moojeni, B. leucurus, e B. atrox. Estes grupos coincidem com aqueles obtidos por outros métodos indicando que os marcadores de RAPD podem ser considerados ferramentas úteis para a avaliação de relações a nível interespecífico.
topic Bothrops
RAPD
molecular markers
genetic relationship
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005
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