Neuroamines — regulators of local processes at bone marrow autotransplantation

• Main Author: Vorobyova O.V.
• Format: Article
• Language: Russian
• Published: Saratov State Medical University 2015-12-01
• Series: Саратовский научно-медицинский журнал
• Subjects: bone marrow autotransplantation; catecholamines; granular luminescent cells; histamine; mastcells; serotonin
• Online Access: http://www.ssmj.ru/system/files/2015_04_524-526.pdf

Purpose: to determine the content of neuroamines (histamine, serotonin, catecholamines) in bioamine structures of bone marrow after autotransplantation. Material and methods. Animals were injected into the tail vein of bone marrow suspension obtained from the femur of the same mouse. It was taken from the femoral bone marrow of 1 ml and placed in 2 ml of physiological saline and thoroughly stirred. 1 ml of bone marrow suspension was injected into the tail vein. Cryostat sections were treated with luminescent-histochemical methods. Results. After bone marrow autotransplantation marked changes were observed in the neurotransmitters of the bone marrow — an increasing number of granular luminescent cells decrease in the amount of granules in them, and a decrease in the number of mast cells because of their degranulation. A weak luminescence was determined in nuclei in neutrophils. Perhaps this proves the activation of the immune response. Conclusion. The bone marrow autotransplantation leads to a redistribution of the structures of the bone marrow of histamine, catecholamines and serotonin, which changes the direction of cytodifferentiation, the content of neuroamines in granular luminescent cells and mast cells (cells of autonomic regulation).