Correlation of intestinal flora with serum glucagon-like peptide-1 level in patients with nonalcoholic fatty liver disease

• Main Author: LIU Zhiping
• Format: Article
• Language: zho
• Published: Editorial Department of Journal of Clinical Hepatology 2020-01-01
• Series: Linchuang Gandanbing Zazhi
• Online Access: http://www.lcgdbzz.org/qk_content.asp?id=10468

ObjectiveTo investigate the correlation of intestinal dysbacteriosis with serum glucagon-like peptide-1 (GLP-1) level in patients with nonalcoholic fatty liver disease (NAFLD). MethodsA total of 116 patients with NAFLD who attended Wuhan Hankou Hospital from January to December 2018, were enrolled as NAFLD group, and 96 healthy individuals who underwent physical examination in our hospital during the same period of time were enrolled as control group. Fresh feces samples were collected from all subjects and bacterial culture was performed; the change in intestinal flora was analyzed, and the Bifidobacterium-Escherichia coli (B/E) ratio was calculated to evaluate the colonization resistance of intestinal flora. The two groups were compared in terms of the changes in body mass index (BMI), triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), fasting plasma glucose (FPG), fasting insulin (FINS), Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), serum GLP-1 level, number of intestinal bacteria, and B/E ratio. The t-test was used for comparison of continuous data between two groups, and the chi-square test was used for comparison of categorical data between two groups. Pearson correlation analysis and partial correlation analysis were performed. ResultsCompared with the control group, the NAFLD group had significantly higher levels of BMI (24.76±293 kg/m2 vs 22.35±2.54 kg/m2, t=6.327, P＜0.05), TG (1.15±0.65 mmol/L vs 0.93±0.62 mmol/L, t=2.504, P＜0.05), TC (4.06±1.02 mmol/L vs 3.71±1.15 mmol/L, t=2.347, P＜0.05), LDL-C (2.76±0.93 mmol/L vs 2.33±0.87 mmol/L, t=3450, P＜0.05), FINS (15.17±7.39mIU/Lvs12.74±6.81 mIU/L, t=2.469, P＜0.05), HOMA-IR (2.58±1.38vs2.14±125, t=2.411, P＜0.05), Enterobacter (9.56±1.85 log cfu/g vs 8.38±1.92 log cfu/g, t=4.544, P＜0.05）, Enterococcus (8.12±1.47 log cfu/g vs 7.66±1.54 log cfu/g, t=2.219, P＜0.05）, and Staphylococcus (7.54±1.22 log cfu/g vs 7.03±118 log cfu/g, t=3075, P＜0.05）, as well as significantly lower levels of serum GLP-1 (6.95±2.74 pmol/L vs 8.06±2.86 pmol/L, t=-2.878, P＜005), Bifidobacterium (8.87±1.76 log cfu/g vs 9.71±1.86 log cfu/g, t=-3.371, P＜0.05）, Lactobacillus (8.31±1.55 log cfu/g vs 8.97±1.57 log cfu/g, t=-3.068, P＜0.05）, Bacteroides (8.66±1.21 log cfu/g vs 9.23±1.34 log cfu/g, t=-3.251, P＜005）, and B/E ratio (0.96±0.17 vs 1.15±0.15, t=-8.539, P＜0.05). Serum GLP-1 level was positively correlated with B/E ratio (r=0.604, P＜0.05) and was negatively correlated with BMI, TG, TC, LDL-C, and HOMA-IR (r=-0.551, -0.424, -0386, -0.445, and -0.503, all P＜0.05). After the control for BMI, TG, TC, LDL-C, and HOMA-IR, serum GLP-1 level was still positively correlated with B/E ratio (r=0.576, P＜0.05). ConclusionThe reduction in serum GLP-1 level is correlated with intestinal dysbacteriosis in patients with NAFLD.