Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines

Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines

The enzyme 5.1O-methenyltetrahydrofolate synthetase catalyzes the key enzymatic reaction in the pathway by which 5-formyl tetrahydrofolate (leucovorin) enters the folate coenzyme pool. The distribution of this enzyme in different organs and tumor cell lines was studied. This enzyme has a wide distri...

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Main Authors: Li Mingxia, Collier Christine, Gritsman Helena, Bertino Joseph R.
Format: Article
Language:English
Published: De Gruyter 1990-05-01
Series:Pteridines
Online Access:https://doi.org/10.1515/pteridines.1990.2.2.75
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spelling doaj-f5b1f69a6ed54074947ba4d3b75033bf2021-09-05T13:59:58ZengDe GruyterPteridines0933-48072195-47201990-05-0122757910.1515/pteridines.1990.2.2.75Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell LinesLi Mingxia0Collier Christine1Gritsman Helena2Bertino Joseph R.3Cornell University Graduate School of Medical Sciences. Laboratory of Molecular Pharmacology, Memorial Sloan-Kettering Cancer Center, New York. NY. 10021. U.S.A.Cornell University Graduate School of Medical Sciences. Laboratory of Molecular Pharmacology, Memorial Sloan-Kettering Cancer Center, New York. NY. 10021. U.S.A.Cornell University Graduate School of Medical Sciences. Laboratory of Molecular Pharmacology, Memorial Sloan-Kettering Cancer Center, New York. NY. 10021. U.S.A.Cornell University Graduate School of Medical Sciences. Laboratory of Molecular Pharmacology, Memorial Sloan-Kettering Cancer Center, New York. NY. 10021. U.S.A.The enzyme 5.1O-methenyltetrahydrofolate synthetase catalyzes the key enzymatic reaction in the pathway by which 5-formyl tetrahydrofolate (leucovorin) enters the folate coenzyme pool. The distribution of this enzyme in different organs and tumor cell lines was studied. This enzyme has a wide distribution among organs and tumor cell lines with highest levels of activity in liver and kidney. Tumor cell lines also contain significant levels of this enzyme activity. Thus, leucovorin can be converted to active folate coenzyme forms in organs and tumor cells, and does not require conversion in liver alone. The implications of these findings for leucovorin usage as "rescue" after methotrexate treatment or to augment fluoropyrimidine cytotoxicity are discussed.https://doi.org/10.1515/pteridines.1990.2.2.75
collection DOAJ
language English
format Article
sources DOAJ
author Li Mingxia
Collier Christine
Gritsman Helena
Bertino Joseph R.
spellingShingle Li Mingxia
Collier Christine
Gritsman Helena
Bertino Joseph R.
Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines
Pteridines
author_facet Li Mingxia
Collier Christine
Gritsman Helena
Bertino Joseph R.
author_sort Li Mingxia
title Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines
title_short Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines
title_full Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines
title_fullStr Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines
title_full_unstemmed Activity of 5, 10-Methenyltetrahydrofolate Synthetase in Rat Tissues and in Tumor Cell Lines
title_sort activity of 5, 10-methenyltetrahydrofolate synthetase in rat tissues and in tumor cell lines
publisher De Gruyter
series Pteridines
issn 0933-4807
2195-4720
publishDate 1990-05-01
description The enzyme 5.1O-methenyltetrahydrofolate synthetase catalyzes the key enzymatic reaction in the pathway by which 5-formyl tetrahydrofolate (leucovorin) enters the folate coenzyme pool. The distribution of this enzyme in different organs and tumor cell lines was studied. This enzyme has a wide distribution among organs and tumor cell lines with highest levels of activity in liver and kidney. Tumor cell lines also contain significant levels of this enzyme activity. Thus, leucovorin can be converted to active folate coenzyme forms in organs and tumor cells, and does not require conversion in liver alone. The implications of these findings for leucovorin usage as "rescue" after methotrexate treatment or to augment fluoropyrimidine cytotoxicity are discussed.
url https://doi.org/10.1515/pteridines.1990.2.2.75
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AT collierchristine activityof510methenyltetrahydrofolatesynthetaseinrattissuesandintumorcelllines
AT gritsmanhelena activityof510methenyltetrahydrofolatesynthetaseinrattissuesandintumorcelllines
AT bertinojosephr activityof510methenyltetrahydrofolatesynthetaseinrattissuesandintumorcelllines
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