Correlation of Single Nucleotide Polymorphism 35-Kb Upstream of Hla-C and Clinical Profile of Multidrug-Resistant Tuberculosis

• Main Authors: Marwoto, Umi Hani’ Vismayanti Lismana, Afiono Agung Prasetyo, Suradi, Reviono, Harsini
• Format: Article
• Language: English
• Published: JCDR Research and Publications Private Limited 2015-09-01
• Series: Journal of Clinical and Diagnostic Research
• Subjects: human leukocyte antigen c; major histocompatibility complex class i; rs9264942
• Online Access: https://jcdr.net/articles/PDF/6451/14439_CE[Ra1]_F(AK)_PF1(PAK)_PFA(AK)_PF2(PAG).pdf

Introduction: The SNP HLA-C-35 kb (rs9264942) may contribute to the host immune defense mechanism by affecting the cell surface expression pattern of HLA-C and antigen presentation to CD8+ cytotoxic cells. Thus, this SNP may contribute to intracellular multidrug-resistant (MDR)-tuberculosis (TB) infection. Aim: To examine the association between the SNP HLA-C-35 kb (rs9264942) and the clinical profile of MDR-TB infection. Settings and Design: MDR-TB-positive patients were followed from May 2012 to December 2013 to observe the progression of MDR-TB infection. Non-TB individuals and non-MDR-TB individuals were also recruited as controls. Materials and Methods: The patients’ HLA-C-35 kb (rs9264942) status was determined by PCR. Results: The C allele was slightly more frequent in the MDR-TB patients than in the non-MDR TB patients (OR= 1.28; 95% CI: 0.701 – 2.328). The C allele was found to be more frequent in the MDR-TB patients exhibiting pulmonary fibrosis (OR= 2.13; 95% CI: 0.606 – 7.480) or pulmonary infiltrates (OR= 3.17; 95% CI: 0.690 – 14.598) and among the MDR-TB patients who were classified as underweight (OR= 8.00; 95% CI: 1.261 – 50.770). The CC genotype was associated with the treatment after failure of category II group (OR= 4.17; 95% CI: 1.301 – 13.346). Conclusion: The C allele SNP HLA-C-35 kb (rs9264942) may contribute to the clinical profile in MDR-TB infection.