n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway
We elucidated the mechanisms of action of two n-3 PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in Jurkat T-cells. Both DHA and EPA were principally incorporated into phospholipids in the following order: phosphatidylcholine < phosphatidylethanolamine < phosphatidylinosito...
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Elsevier
2005-04-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520340104 |
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doaj-f588bc13e00840cf80ff1d696ad90b1b2021-04-27T04:46:22ZengElsevierJournal of Lipid Research0022-22752005-04-01464752758n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathwayAnne Denys0Aziz Hichami1Naim Akhtar Khan2University of Burgundy, Department of Physiology, Unité Propre de Recherche et de l'Enseignement Supérieur (UPRES) Lipids and Nutrition, Faculty of Life Sciences, Dijon 21000, FranceUniversity of Burgundy, Department of Physiology, Unité Propre de Recherche et de l'Enseignement Supérieur (UPRES) Lipids and Nutrition, Faculty of Life Sciences, Dijon 21000, FranceUniversity of Burgundy, Department of Physiology, Unité Propre de Recherche et de l'Enseignement Supérieur (UPRES) Lipids and Nutrition, Faculty of Life Sciences, Dijon 21000, FranceWe elucidated the mechanisms of action of two n-3 PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in Jurkat T-cells. Both DHA and EPA were principally incorporated into phospholipids in the following order: phosphatidylcholine < phosphatidylethanolamine < phosphatidylinositol/phosphatidylserine. Furthermore, two isoforms of phospholipase A2 (i.e., calcium-dependent and calcium-independent) were implicated in the release of DHA and EPA, respectively, during activation of these cells. The two fatty acids inhibited the phorbol 12-myristate 13-acetate (PMA)-induced plasma membrane translocation of protein kinase C (PKC)-α and -ε. The two n-3 PUFAs also inhibited the nuclear translocation of nuclear factor κB (NF-κB) and the transcription of the interleukin-2 (IL-2) gene in PMA-activated Jurkat T-cells.Together, these results demonstrate that DHA and EPA, being released by two isoforms of phospholipase A2, modulate IL-2 gene expression by exerting their action on two PKC isoforms and NF-κB in Jurkat T-cells.http://www.sciencedirect.com/science/article/pii/S0022227520340104fatty acidsmitogen-activated protein kinasepolyunsaturated fatty acidsnuclear factor κB |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Anne Denys Aziz Hichami Naim Akhtar Khan |
| spellingShingle |
Anne Denys Aziz Hichami Naim Akhtar Khan n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway Journal of Lipid Research fatty acids mitogen-activated protein kinase polyunsaturated fatty acids nuclear factor κB |
| author_facet |
Anne Denys Aziz Hichami Naim Akhtar Khan |
| author_sort |
Anne Denys |
| title |
n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway |
| title_short |
n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway |
| title_full |
n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway |
| title_fullStr |
n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway |
| title_full_unstemmed |
n-3 PUFAs modulate T-cell activation via protein kinase C-α and -ε and the NF-κB signaling pathway |
| title_sort |
n-3 pufas modulate t-cell activation via protein kinase c-α and -ε and the nf-κb signaling pathway |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
2005-04-01 |
| description |
We elucidated the mechanisms of action of two n-3 PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in Jurkat T-cells. Both DHA and EPA were principally incorporated into phospholipids in the following order: phosphatidylcholine < phosphatidylethanolamine < phosphatidylinositol/phosphatidylserine. Furthermore, two isoforms of phospholipase A2 (i.e., calcium-dependent and calcium-independent) were implicated in the release of DHA and EPA, respectively, during activation of these cells. The two fatty acids inhibited the phorbol 12-myristate 13-acetate (PMA)-induced plasma membrane translocation of protein kinase C (PKC)-α and -ε. The two n-3 PUFAs also inhibited the nuclear translocation of nuclear factor κB (NF-κB) and the transcription of the interleukin-2 (IL-2) gene in PMA-activated Jurkat T-cells.Together, these results demonstrate that DHA and EPA, being released by two isoforms of phospholipase A2, modulate IL-2 gene expression by exerting their action on two PKC isoforms and NF-κB in Jurkat T-cells. |
| topic |
fatty acids mitogen-activated protein kinase polyunsaturated fatty acids nuclear factor κB |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520340104 |
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