Spatial reconstruction of myocardial connective tissue component in rat

• Main Author: Gorbunov A.A.
• Format: Article
• Language: Russian
• Published: Ministry of Health of Ukraine. Dnipropetrovsk Medical Academy 2008-01-01
• Series: Морфологія
• Subjects: myocardium; connective tissue component; reconstruction; serial cuts; three-dimensional model
• Online Access: http://www.morphology.dp.ua/_pub/MORPHO-2008-02-02/08gaakmk.pdf

The aim of the study was building up a three-dimensional model of myocardium fragment, visualization of spatial organization its muscular and connective tissue components. The hearts of adult white rats served as a material for the study. Standard histological methods have been used. Specimen staining has been done by van Gison method, phospho-wolframic hematoxylin, and iron hematoxylin by Heidenhein. Based on serial consequential cuts microphotos a spatial reconstruction of myocardium fragment has been built up. Special software have been used – Amira for microscopy. The 3D model obtained allowed to evaluate visually the correlation of muscular and connective tissue components, as well as to render volumetric myocardial characteristics that have been obtained by stereological approach. The model of myocardium could be used in other researches for the demonstration of data obtained.