Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

Introduction: The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes in biological samples of th...

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Main Authors: Kędrak-Jabłońska Agnieszka, Budniak Sylwia, Krupa Marek, Szczawińska Anna, Reksa Monika, Szulowski Krzysztof, Iwaniak Wojciech
Format: Article
Language:English
Published: Sciendo 2017-12-01
Series:Journal of Veterinary Research
Subjects:
listeria monocytogenes
real-time pcr
sybr green i
taqman probe
Online Access:https://doi.org/10.1515/jvetres-2017-0069

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