Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

Introduction: The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes in biological samples of th...

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Main Authors: Kędrak-Jabłońska Agnieszka, Budniak Sylwia, Krupa Marek, Szczawińska Anna, Reksa Monika, Szulowski Krzysztof, Iwaniak Wojciech
Format: Article
Language:English
Published: Sciendo 2017-12-01
Series:Journal of Veterinary Research
Subjects:
listeria monocytogenes
real-time pcr
sybr green i
taqman probe
Online Access:https://doi.org/10.1515/jvetres-2017-0069
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spelling doaj-f560d88fe3e84c3b95503af326f3db792021-09-06T19:40:55ZengSciendoJournal of Veterinary Research2450-86082017-12-0161442743210.1515/jvetres-2017-0069jvetres-2017-0069Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRsKędrak-Jabłońska Agnieszka0Budniak Sylwia1Krupa Marek2Szczawińska Anna3Reksa Monika4Szulowski Krzysztof5Iwaniak Wojciech6Department of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandDepartment of Microbiology, National Veterinary Research Institute, 24-100Pulawy, PolandIntroduction: The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes in biological samples of the liver, brain, and blood.https://doi.org/10.1515/jvetres-2017-0069listeria monocytogenesreal-time pcrsybr green itaqman probe
collection DOAJ
language English
format Article
sources DOAJ
author Kędrak-Jabłońska Agnieszka
Budniak Sylwia
Krupa Marek
Szczawińska Anna
Reksa Monika
Szulowski Krzysztof
Iwaniak Wojciech
spellingShingle Kędrak-Jabłońska Agnieszka
Budniak Sylwia
Krupa Marek
Szczawińska Anna
Reksa Monika
Szulowski Krzysztof
Iwaniak Wojciech
Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs
Journal of Veterinary Research
listeria monocytogenes
real-time pcr
sybr green i
taqman probe
author_facet Kędrak-Jabłońska Agnieszka
Budniak Sylwia
Krupa Marek
Szczawińska Anna
Reksa Monika
Szulowski Krzysztof
Iwaniak Wojciech
author_sort Kędrak-Jabłońska Agnieszka
title Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs
title_short Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs
title_full Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs
title_fullStr Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs
title_full_unstemmed Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs
title_sort detection of listeria spp. and listeria monocytogenes in biological samples by sybr green i and taqman probe-based real-time pcrs
publisher Sciendo
series Journal of Veterinary Research
issn 2450-8608
publishDate 2017-12-01
description Introduction: The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes in biological samples of the liver, brain, and blood.
topic listeria monocytogenes
real-time pcr
sybr green i
taqman probe
url https://doi.org/10.1515/jvetres-2017-0069
work_keys_str_mv AT kedrakjabłonskaagnieszka detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
AT budniaksylwia detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
AT krupamarek detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
AT szczawinskaanna detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
AT reksamonika detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
AT szulowskikrzysztof detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
AT iwaniakwojciech detectionoflisteriasppandlisteriamonocytogenesinbiologicalsamplesbysybrgreeniandtaqmanprobebasedrealtimepcrs
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