A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury
Hydrogen sulfide (H2S) has been recognized as an important gasotransmitter exerting various physiological effects, especially in the cardiovascular system. Herein we investigated the cardioprotective effects of a novel long-term and slow-releasing H2S donor, DATS-MSN, using in vivo myocardial ischem...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2017-06-01
|
Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-017-03941-0 |
id |
doaj-f5458918a7e14749bc0e84cf099e6a9f |
---|---|
record_format |
Article |
spelling |
doaj-f5458918a7e14749bc0e84cf099e6a9f2020-12-08T02:43:59ZengNature Publishing GroupScientific Reports2045-23222017-06-017111310.1038/s41598-017-03941-0A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion InjuryXiaotian Sun0Wenshuo Wang1Jing Dai2Sheng Jin3Jiechun Huang4Changfa Guo5Chunsheng Wang6Liewen Pang7Yiqing Wang8Department of Cardiothoracic Surgery, Huashan Hospital of Fudan UniversityDepartment of Cardiac Surgery, Zhongshan Hospital of Fudan University and Shanghai Institute of Cardiovascular DiseasesDepartment of Cadres Health Care, Third Hospital of ShijiazhuangDepartment of Physiology, Hebei Medical UniversityDepartment of Cardiothoracic Surgery, Huashan Hospital of Fudan UniversityDepartment of Cardiac Surgery, Zhongshan Hospital of Fudan University and Shanghai Institute of Cardiovascular DiseasesDepartment of Cardiac Surgery, Zhongshan Hospital of Fudan University and Shanghai Institute of Cardiovascular DiseasesDepartment of Cardiothoracic Surgery, Huashan Hospital of Fudan UniversityDepartment of Cardiothoracic Surgery, Huashan Hospital of Fudan UniversityHydrogen sulfide (H2S) has been recognized as an important gasotransmitter exerting various physiological effects, especially in the cardiovascular system. Herein we investigated the cardioprotective effects of a novel long-term and slow-releasing H2S donor, DATS-MSN, using in vivo myocardial ischemia/reperfusion (I/R) models and in vitro hypoxia/reoxygenation cardiomyocyte models. Unlike the instant-releasing pattern of sodium hydrosulphide (NaHS), the release of H2S from DATS-MSN was quite slow and continuous both in the cell culture medium and in rat plasma (elevated H2S concentrations during 24 h and 72 h reperfusion). Correspondingly, DATS-MSN demonstrated superior cardioprotective effects over NaHS in I/R models, which were associated with greater survival rates, reduced CK-MB and troponin I levels, decreased cardiomyocyte apoptosis index, increased antioxidant enzyme activities, inhibited myocardial inflammation, greater reduction in the infarct area and preserved cardiac ejection fraction. Some of these effects of DATS-MSN were also found to be superior to classic slow-releasing H2S donor, GYY4137. In in vitro experiments, cardiomyocytes injury was also found to be relived with the use of DATS-MSN compared to NaHS after the hypoxia/reoxygenation processes. The present work provides a novel long-term and slow-releasing H2S donor and an insight into how the release patterns of H2S donors affect its physiological functionality.https://doi.org/10.1038/s41598-017-03941-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaotian Sun Wenshuo Wang Jing Dai Sheng Jin Jiechun Huang Changfa Guo Chunsheng Wang Liewen Pang Yiqing Wang |
spellingShingle |
Xiaotian Sun Wenshuo Wang Jing Dai Sheng Jin Jiechun Huang Changfa Guo Chunsheng Wang Liewen Pang Yiqing Wang A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury Scientific Reports |
author_facet |
Xiaotian Sun Wenshuo Wang Jing Dai Sheng Jin Jiechun Huang Changfa Guo Chunsheng Wang Liewen Pang Yiqing Wang |
author_sort |
Xiaotian Sun |
title |
A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury |
title_short |
A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury |
title_full |
A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury |
title_fullStr |
A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury |
title_full_unstemmed |
A Long-Term and Slow-Releasing Hydrogen Sulfide Donor Protects against Myocardial Ischemia/Reperfusion Injury |
title_sort |
long-term and slow-releasing hydrogen sulfide donor protects against myocardial ischemia/reperfusion injury |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-06-01 |
description |
Hydrogen sulfide (H2S) has been recognized as an important gasotransmitter exerting various physiological effects, especially in the cardiovascular system. Herein we investigated the cardioprotective effects of a novel long-term and slow-releasing H2S donor, DATS-MSN, using in vivo myocardial ischemia/reperfusion (I/R) models and in vitro hypoxia/reoxygenation cardiomyocyte models. Unlike the instant-releasing pattern of sodium hydrosulphide (NaHS), the release of H2S from DATS-MSN was quite slow and continuous both in the cell culture medium and in rat plasma (elevated H2S concentrations during 24 h and 72 h reperfusion). Correspondingly, DATS-MSN demonstrated superior cardioprotective effects over NaHS in I/R models, which were associated with greater survival rates, reduced CK-MB and troponin I levels, decreased cardiomyocyte apoptosis index, increased antioxidant enzyme activities, inhibited myocardial inflammation, greater reduction in the infarct area and preserved cardiac ejection fraction. Some of these effects of DATS-MSN were also found to be superior to classic slow-releasing H2S donor, GYY4137. In in vitro experiments, cardiomyocytes injury was also found to be relived with the use of DATS-MSN compared to NaHS after the hypoxia/reoxygenation processes. The present work provides a novel long-term and slow-releasing H2S donor and an insight into how the release patterns of H2S donors affect its physiological functionality. |
url |
https://doi.org/10.1038/s41598-017-03941-0 |
work_keys_str_mv |
AT xiaotiansun alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT wenshuowang alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT jingdai alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT shengjin alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT jiechunhuang alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT changfaguo alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT chunshengwang alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT liewenpang alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT yiqingwang alongtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT xiaotiansun longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT wenshuowang longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT jingdai longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT shengjin longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT jiechunhuang longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT changfaguo longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT chunshengwang longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT liewenpang longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury AT yiqingwang longtermandslowreleasinghydrogensulfidedonorprotectsagainstmyocardialischemiareperfusioninjury |
_version_ |
1724393420081856512 |