A Crude Extract Preparation and Optimization from a Genomically Engineered <i>Escherichia coli</i> for the Cell-Free Protein Synthesis System: Practical Laboratory Guideline

A Crude Extract Preparation and Optimization from a Genomically Engineered <i>Escherichia coli</i> for the Cell-Free Protein Synthesis System: Practical Laboratory Guideline

With the advancement of synthetic biology, the cell-free protein synthesis (CFPS) system has been receiving the spotlight as a versatile toolkit for engineering natural and unnatural biological systems. The CFPS system reassembles the materials necessary for transcription and translation and recreat...

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Bibliographic Details
Main Authors: Jeehye Kim, Caroline E. Copeland, Sahana R. Padumane, Yong-Chan Kwon
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Methods and Protocols
Subjects:
cell-free protein synthesis
E. coli crude extract preparation
genomically engineered E. coli
sonication
Online Access:https://www.mdpi.com/2409-9279/2/3/68

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