Turn-On Fluorescence Detection of Glutathione Based on o-Phthaldialdehyde-Assisted SiO2 Particles

• Main Authors: Polonca Nedeljko, Matejka Turel, Aleksandra Lobnik
• Format: Article
• Language: English
• Published: Hindawi Limited 2018-01-01
• Series: Journal of Sensors
• Online Access: http://dx.doi.org/10.1155/2018/1692702

We report about the fluorometric method for the determination of glutathione (GSH) in dietary supplements. The fluorometric assay is based on the use of functionalized silica particles (SiO2-SH) in combination with o-phthaldialdehyde (OPA). The fluorescence excitation and emission wavelengths were located at 340 nm and 430 nm. The relative fluorescence intensity was linear in the concentration range between 1.0 × 10−7 mol/L and 1.0 × 10−5 mol/L (M) with the detection limit of 3.4 × 10−7 M. The fluorescent silica particle-based sensor was successfully employed for the determination of GSH in dietary supplements with excellent recoveries. The proposed sensor may pave a new way for fluorescence sensing of other substances in food samples.