An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual

An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual

We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent...

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Main Authors: Lijing Wang, Hongjun Zhao, Junjiao Wu, Jie Ren, Hui Luo, Xiaoxia Zuo, Qiong Chen, Yu Tang
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001823
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spelling doaj-f517b0b84ee149a3956a2caadd151b342021-05-30T04:41:38ZengElsevierStem Cell Research1873-50612021-05-0153102336An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individualLijing Wang0Hongjun Zhao1Junjiao Wu2Jie Ren3Hui Luo4Xiaoxia Zuo5Qiong Chen6Yu Tang7Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha 410008, China; Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, ChinaDepartment of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Key Laboratory of Hunan Province in Neurodegenerative Disorders, Central South University, Changsha, Hunan 410008, China; Corresponding author at: Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China.We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent as determined by immunostaining and expression of pluripotency markers. They were further induced as embryoid bodies in vitro and teratomas in vivo, reminiscent of their full capacity of differentiating into three germ layers. They are with a normal karyotype and genetically identical to donor fibroblasts. This iPSC line provides excellent cell sources for studying and modeling human-specific physiology.http://www.sciencedirect.com/science/article/pii/S1873506121001823
collection DOAJ
language English
format Article
sources DOAJ
author Lijing Wang
Hongjun Zhao
Junjiao Wu
Jie Ren
Hui Luo
Xiaoxia Zuo
Qiong Chen
Yu Tang
spellingShingle Lijing Wang
Hongjun Zhao
Junjiao Wu
Jie Ren
Hui Luo
Xiaoxia Zuo
Qiong Chen
Yu Tang
An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual
Stem Cell Research
author_facet Lijing Wang
Hongjun Zhao
Junjiao Wu
Jie Ren
Hui Luo
Xiaoxia Zuo
Qiong Chen
Yu Tang
author_sort Lijing Wang
title An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual
title_short An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual
title_full An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual
title_fullStr An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual
title_full_unstemmed An induced pluripotent stem cell line (CSUi004-A) from skin fibroblasts of a healthy individual
title_sort induced pluripotent stem cell line (csui004-a) from skin fibroblasts of a healthy individual
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description We established an induced pluripotent stem cell (iPSC) reprogrammed from dermal fibroblasts of a 53-year-old healthy man. Retroviruses expressing Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) were employed to initiate the reprogramming and were silenced in iPSCs. The generated iPSCs were pluripotent as determined by immunostaining and expression of pluripotency markers. They were further induced as embryoid bodies in vitro and teratomas in vivo, reminiscent of their full capacity of differentiating into three germ layers. They are with a normal karyotype and genetically identical to donor fibroblasts. This iPSC line provides excellent cell sources for studying and modeling human-specific physiology.
url http://www.sciencedirect.com/science/article/pii/S1873506121001823
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