Preparation of Organic Seleno cystine Using Locally Isolated Yeast

selenium is a toxic inorganic form at very low concentration to the life, while theorganic-selenium compound sareappre ciableinterest and various of them haveessentialrolesinnutritionalscienceandcellbiochemistry.Selenium-enrichedyeast(Seyeast)isapublicformofseleniumusedtoadditionaldietaryintakeofthi...

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Main Author: Fouad Houssein Kamel
Format: Article
Language:Arabic
Published: University of Al-Qadisiya 2018-05-01
Series:Al-Qadisiyah Journal For Agriculture Sciences
Online Access:http://qu.edu.iq/jouagr/index.php/QJAS/article/view/47
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spelling doaj-f4ad031308254bdbac350838078529d72020-11-25T01:51:15ZaraUniversity of Al-QadisiyaAl-Qadisiyah Journal For Agriculture Sciences2077-58222618-14792018-05-018147Preparation of Organic Seleno cystine Using Locally Isolated YeastFouad Houssein Kamel0Medical Technical Institute, Erbil Polytechnic University ,Erbilselenium is a toxic inorganic form at very low concentration to the life, while theorganic-selenium compound sareappre ciableinterest and various of them haveessentialrolesinnutritionalscienceandcellbiochemistry.Selenium-enrichedyeast(Seyeast)isapublicformofseleniumusedtoadditionaldietaryintakeofthisessentialtracemineral.Inthisstudy,weprepareanorganicselenocystinebyusinglocallyisolatedbakeryyeast(Saccharomycescerevisiae).Anovellocallyprepareddateextractmediaenrichedby0.2%potassiumphosphate(KH2PO4),0.6%ammoniumsulfatewasadoptedasalternativeculturemedia.Seleniumsaltwas used in differentconcentrations(30,60,120and240μg/mL),whichwereaddedtotheyeastculturemedia.Whilethebestconcentrationofseleniumaddedwas30μg/mL,itachievedoptimalconditionsforthegrowthof red coloryea stidentical to the standard.Theorganicselenocystine ereanalyzed by High Performance LiquidChromatography (HPLC) and Atomic Absorption Spectrophotometer (AAS) comparing with standard product obtained from Sigma. Results confirmedtheformationofsimilarselenocystineproductshttp://qu.edu.iq/jouagr/index.php/QJAS/article/view/47
collection DOAJ
language Arabic
format Article
sources DOAJ
author Fouad Houssein Kamel
spellingShingle Fouad Houssein Kamel
Preparation of Organic Seleno cystine Using Locally Isolated Yeast
Al-Qadisiyah Journal For Agriculture Sciences
author_facet Fouad Houssein Kamel
author_sort Fouad Houssein Kamel
title Preparation of Organic Seleno cystine Using Locally Isolated Yeast
title_short Preparation of Organic Seleno cystine Using Locally Isolated Yeast
title_full Preparation of Organic Seleno cystine Using Locally Isolated Yeast
title_fullStr Preparation of Organic Seleno cystine Using Locally Isolated Yeast
title_full_unstemmed Preparation of Organic Seleno cystine Using Locally Isolated Yeast
title_sort preparation of organic seleno cystine using locally isolated yeast
publisher University of Al-Qadisiya
series Al-Qadisiyah Journal For Agriculture Sciences
issn 2077-5822
2618-1479
publishDate 2018-05-01
description selenium is a toxic inorganic form at very low concentration to the life, while theorganic-selenium compound sareappre ciableinterest and various of them haveessentialrolesinnutritionalscienceandcellbiochemistry.Selenium-enrichedyeast(Seyeast)isapublicformofseleniumusedtoadditionaldietaryintakeofthisessentialtracemineral.Inthisstudy,weprepareanorganicselenocystinebyusinglocallyisolatedbakeryyeast(Saccharomycescerevisiae).Anovellocallyprepareddateextractmediaenrichedby0.2%potassiumphosphate(KH2PO4),0.6%ammoniumsulfatewasadoptedasalternativeculturemedia.Seleniumsaltwas used in differentconcentrations(30,60,120and240μg/mL),whichwereaddedtotheyeastculturemedia.Whilethebestconcentrationofseleniumaddedwas30μg/mL,itachievedoptimalconditionsforthegrowthof red coloryea stidentical to the standard.Theorganicselenocystine ereanalyzed by High Performance LiquidChromatography (HPLC) and Atomic Absorption Spectrophotometer (AAS) comparing with standard product obtained from Sigma. Results confirmedtheformationofsimilarselenocystineproducts
url http://qu.edu.iq/jouagr/index.php/QJAS/article/view/47
work_keys_str_mv AT fouadhousseinkamel preparationoforganicselenocystineusinglocallyisolatedyeast
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