Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)

We examined the potential utility of museum specimens as a source for genetic analysis of fairy shrimp. Because of loss of their vernal pool habitat, some fairy shrimp (including Branchinecta sandiegonensis and B. lynchi) are listed as threatened or endangered in Southern California by the United St...

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Main Authors: Adam Wall, Daniel Campo, Regina Wetzer
Format: Article
Language:English
Published: Pensoft Publishers 2014-11-01
Series:ZooKeys
Online Access:http://zookeys.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=4245
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spelling doaj-f4ac2bb7c6484d85b7ef01acb66390e82020-11-24T22:59:59ZengPensoft PublishersZooKeys1313-29891313-29702014-11-0145711410.3897/zookeys.457.68224245Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)Adam WallDaniel CampoRegina WetzerWe examined the potential utility of museum specimens as a source for genetic analysis of fairy shrimp. Because of loss of their vernal pool habitat, some fairy shrimp (including Branchinecta sandiegonensis and B. lynchi) are listed as threatened or endangered in Southern California by the United States Fish and Wildlife Service. Management of those species requires extensive population genetics studies and the resolution of important genetic complexity (e.g. possible hybridization between endangered and non-endangered species). Regulations mandating deposition of specimens of listed species have resulted in thousands of specimens accessioned into the Natural History Museum of Los Angeles County that have been preserved in a variety of solutions. We subsampled those specimens, as well as other Anostraca with known collection and preservation histories, to test their potential for genetic analysis by attempting DNA extraction and amplification for mt16SrDNA. Fixation and preservation in not denatured ethanol had a far greater sequencing success rate than other (and unknown) fixatives and preservatives. To maximize scientific value we recommend field preservation in 95% not denatured ethanol (or, if pure ethanol is unavailable, high-proof drinking spirits, e.g. Everclear™, or 151 proof white rum), followed by storage in 95% not denatured ethanol.http://zookeys.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=4245
collection DOAJ
language English
format Article
sources DOAJ
author Adam Wall
Daniel Campo
Regina Wetzer
spellingShingle Adam Wall
Daniel Campo
Regina Wetzer
Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)
ZooKeys
author_facet Adam Wall
Daniel Campo
Regina Wetzer
author_sort Adam Wall
title Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)
title_short Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)
title_full Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)
title_fullStr Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)
title_full_unstemmed Genetic utility of natural history museum specimens: endangered fairy shrimp (Branchiopoda, Anostraca)
title_sort genetic utility of natural history museum specimens: endangered fairy shrimp (branchiopoda, anostraca)
publisher Pensoft Publishers
series ZooKeys
issn 1313-2989
1313-2970
publishDate 2014-11-01
description We examined the potential utility of museum specimens as a source for genetic analysis of fairy shrimp. Because of loss of their vernal pool habitat, some fairy shrimp (including Branchinecta sandiegonensis and B. lynchi) are listed as threatened or endangered in Southern California by the United States Fish and Wildlife Service. Management of those species requires extensive population genetics studies and the resolution of important genetic complexity (e.g. possible hybridization between endangered and non-endangered species). Regulations mandating deposition of specimens of listed species have resulted in thousands of specimens accessioned into the Natural History Museum of Los Angeles County that have been preserved in a variety of solutions. We subsampled those specimens, as well as other Anostraca with known collection and preservation histories, to test their potential for genetic analysis by attempting DNA extraction and amplification for mt16SrDNA. Fixation and preservation in not denatured ethanol had a far greater sequencing success rate than other (and unknown) fixatives and preservatives. To maximize scientific value we recommend field preservation in 95% not denatured ethanol (or, if pure ethanol is unavailable, high-proof drinking spirits, e.g. Everclear™, or 151 proof white rum), followed by storage in 95% not denatured ethanol.
url http://zookeys.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=4245
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