Typing class I HLA-A gene using a nested PCR-RFLP procedure

• Main Authors: E.C. Castelli, D.S. Gil, L.C.S. Veiga, J.L.V. de Camargo
• Format: Article
• Language: English
• Published: Associação Brasileira de Divulgação Científica 2005-06-01
• Series: Brazilian Journal of Medical and Biological Research
• Subjects: Human leukocyte antigen-A; HLA-A; Typing; RFLP; Polymorphism; Major histocompatibility complex
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600004

In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95%. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.