The Influence of Repeated Blood Withdrawals before Surgery on Clinical Outcome

• Main Authors: Helena Živná, Pavel Živný, Vladimír Palička, Eva Šimáková, Vít Řeháček
• Format: Article
• Language: English
• Published: Karolinum Press 2007-01-01
• Series: Acta Medica
• Subjects: Preconditioning; Iron; Respiratory burst; Blood donor; Rat; Elective surgery
• Online Access: https://actamedica.lfhk.cuni.cz/50/2/0129/

The aim of this study was to find the influence of blood withdrawals and diet iron on elective surgery. Male Wistar rats (n=24) were divided: 1. group (SLD) ate standard laboratory diet (SLD), 2. group (FE) an iron enriched diet (FE) with one blood withdrawal after 9 weeks. 3. group (SLD-w) SLD and 4. group (FE-w) ate the FE diet; with 9 withdrawals once a week. The rats were sacrificed 18 hour after partial hepatectomy (PH) in the 10th week. Liver DNA synthesis (3H-thymidin – kBq/mg DNA) was performed. Serum hepcidin (pg/ml), iron concentration, respiratory burst of polymorfonucleares (RB, spontaneous; stimulated, %), count of blood cells were determined. FE-w had a higher (2.36±0.36) liver DNA synthesis after PH vs. SLD (1.21±0.49). Higher hemoglobin in erythrocytes (pg) was in FE-w and SLD-w vs. FE and SLD. PMN count in SLD-w, FE-w increased vs. SLD, FE. Hepcidin after PH decreased in SLD (78.0), FE (68.0), FE-w (97.0), but increased in SLD-w (217). Serum iron increased in SLD-w. RB after PH increased in FE-w (4.5; 47.6) vs. SLD (1.15; 29.1), FE (3.20;17.8), SLD-w (3.30;13.7). Conclusions: The iron diet with stimulation of haematopoesis by withdrawals improves an organism’s condition expressed as better response to elective surgery and better PMN functions.