Propagación in vitro de material seleccionado de Tabebuia rosea (Bertol.) DC. (Ocobo) y Cordia alliodora (Ruiz & Pav.) Oken (Nogal Cafetero)*
This work was aimed at multiplying and maintaining in vitro plants from seedlings from five Tabebuia rosea (large spreading cedar tree) from and one Cordia alliodora (walnut tree used for providing shade for coffee plantations). Apical leaf buds were excised from mother plants (MP) and cultured in v...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad Nacional de Colombia
2007-02-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/484 |
| Summary: | This work was aimed at multiplying and maintaining in vitro plants from seedlings from five Tabebuia rosea (large spreading cedar tree) from and one Cordia alliodora (walnut tree used for providing shade for coffee plantations). Apical leaf buds were excised from mother plants (MP) and cultured in vitro until 1480 plants had been produced from the two species. The hardening process was carried out in greenhouse conditions using humid flow-chambers at Geoambiente Ltda's Bambusa experimental station in Pacho, Cundinamarca. A database was created using Microsoft Office Access software for handling information required for monitoring and evaluating each plant's behaviour during the in vitro culture process by calculating the rates of multiplication (RM) and loss (RL). The plants so produced will be used by Conif for field trials and evaluation purposes. This material will then be multiplied by vegetative propagation and used for clone and molecular testing analysis.
Key words: in vitro collection and propagation, Tabebuia rosea, Cordia alliodora. |
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| ISSN: | 0123-3475 1909-8758 |