Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway

Abstract Objective Vascular calcification is prevalent in the aging population, as we know that arterial calcification is associated with aging. Recent studies have demonstrated that carnosine, a naturally occurring dipeptide, performs the treatment of aging‐related diseases, such as atherosclerosis...

Full description

Bibliographic Details
Main Authors:	Yi Huang, Jinli Wang, Mandi Luo, Dan Yan, Cuntai Zhang
Format:	Article
Language:	English
Published:	Wiley 2020-09-01
Series:	Aging Medicine
Subjects:	calcification carnosine vascular smooth muscle cells
Online Access:	https://doi.org/10.1002/agm2.12125

id	doaj-f47937488dbe4a0f9bafc1f81231540e
record_format	Article
spelling	doaj-f47937488dbe4a0f9bafc1f81231540e2021-05-02T19:03:49ZengWileyAging Medicine2475-03602020-09-013315315810.1002/agm2.12125Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathwayYi Huang0Jinli Wang1Mandi Luo2Dan Yan3Cuntai Zhang4Department of Geriatrics Institute of Gerontology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Geriatrics Institute of Gerontology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Geriatrics Institute of Gerontology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Geriatrics Institute of Gerontology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaDepartment of Geriatrics Institute of Gerontology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan ChinaAbstract Objective Vascular calcification is prevalent in the aging population, as we know that arterial calcification is associated with aging. Recent studies have demonstrated that carnosine, a naturally occurring dipeptide, performs the treatment of aging‐related diseases, such as atherosclerosis and type 2 diabetes. Here, we investigated the role of carnosine in a calcification model of vascular smooth muscle cells (VSMCs). Methods In this research, we used an in vitro model of VSMC calcification to investigate the role of carnosine in the progression of rat VSMC calcification. Results Carnosine treatment attenuated calcium deposition in a dose‐dependent manner, detected by Alizarin Red S staining and calcium content assay. Carnosine also reduced the protein level of Runx2, bone morphogenetic protein 2 (BMP‐2), and cellular reactive oxygen species (ROS) production. Further, carnosine inhibited the activation of the mammalian target of rapamycin (mTOR) pathway. Conclusion Carnosine attenuated the VSMC calcification via inhibition of osteoblastic transdifferentiation and the mTOR signaling pathway.https://doi.org/10.1002/agm2.12125calcificationcarnosinevascular smooth muscle cells
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Yi Huang Jinli Wang Mandi Luo Dan Yan Cuntai Zhang
spellingShingle	Yi Huang Jinli Wang Mandi Luo Dan Yan Cuntai Zhang Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway Aging Medicine calcification carnosine vascular smooth muscle cells
author_facet	Yi Huang Jinli Wang Mandi Luo Dan Yan Cuntai Zhang
author_sort	Yi Huang
title	Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway
title_short	Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway
title_full	Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway
title_fullStr	Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway
title_full_unstemmed	Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway
title_sort	carnosine attenuates vascular smooth muscle cells calcification through mtor signaling pathway
publisher	Wiley
series	Aging Medicine
issn	2475-0360
publishDate	2020-09-01
description	Abstract Objective Vascular calcification is prevalent in the aging population, as we know that arterial calcification is associated with aging. Recent studies have demonstrated that carnosine, a naturally occurring dipeptide, performs the treatment of aging‐related diseases, such as atherosclerosis and type 2 diabetes. Here, we investigated the role of carnosine in a calcification model of vascular smooth muscle cells (VSMCs). Methods In this research, we used an in vitro model of VSMC calcification to investigate the role of carnosine in the progression of rat VSMC calcification. Results Carnosine treatment attenuated calcium deposition in a dose‐dependent manner, detected by Alizarin Red S staining and calcium content assay. Carnosine also reduced the protein level of Runx2, bone morphogenetic protein 2 (BMP‐2), and cellular reactive oxygen species (ROS) production. Further, carnosine inhibited the activation of the mammalian target of rapamycin (mTOR) pathway. Conclusion Carnosine attenuated the VSMC calcification via inhibition of osteoblastic transdifferentiation and the mTOR signaling pathway.
topic	calcification carnosine vascular smooth muscle cells
url	https://doi.org/10.1002/agm2.12125
work_keys_str_mv	AT yihuang carnosineattenuatesvascularsmoothmusclecellscalcificationthroughmtorsignalingpathway AT jinliwang carnosineattenuatesvascularsmoothmusclecellscalcificationthroughmtorsignalingpathway AT mandiluo carnosineattenuatesvascularsmoothmusclecellscalcificationthroughmtorsignalingpathway AT danyan carnosineattenuatesvascularsmoothmusclecellscalcificationthroughmtorsignalingpathway AT cuntaizhang carnosineattenuatesvascularsmoothmusclecellscalcificationthroughmtorsignalingpathway
_version_	1721488709255168000

Carnosine attenuates vascular smooth muscle cells calcification through mTOR signaling pathway

Similar Items