The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction
Enzyme hydrolysis optimisation of cell-wall polysaccharides of pumpkin seed with celluloses and pectinases and their mixture, in the relation to enhanced protein extraction was the objective of this study. The individual and combined effects of cell-wall-degrading enzyme activities on protein extrac...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Technology, Novi Sad
2006-01-01
|
| Series: | Acta Periodica Technologica |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2006/1450-71880637163P.pdf |
| id |
doaj-f4513017a0f34cfc997b8013b2b8631e |
|---|---|
| record_format |
Article |
| spelling |
doaj-f4513017a0f34cfc997b8013b2b8631e2020-11-25T00:19:34ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882006-01-0120063716317010.2298/APT0637163PThe use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extractionPeričin Draginja M.Radulović Ljiljana M.Trivić Svetlana Z.Dimić Etelka B.Enzyme hydrolysis optimisation of cell-wall polysaccharides of pumpkin seed with celluloses and pectinases and their mixture, in the relation to enhanced protein extraction was the objective of this study. The individual and combined effects of cell-wall-degrading enzyme activities on protein extraction combined with other process parameters: enzyme concentration and time of hydrolysis were evaluated by the Response Surface Methodology (RSM). The optimal value protein isolate yield (9 g of soluble protein from J 00 g pumpkin seed) is achieved under the following conditions: 2% enzymes (celluloses), reaction time 300 min, pH = 5.0, temperature 45°C. http://www.doiserbia.nb.rs/img/doi/1450-7188/2006/1450-71880637163P.pdfCucurbita pepoprotein isolatesenyzmatic extractionpectinasecellulase |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Peričin Draginja M. Radulović Ljiljana M. Trivić Svetlana Z. Dimić Etelka B. |
| spellingShingle |
Peričin Draginja M. Radulović Ljiljana M. Trivić Svetlana Z. Dimić Etelka B. The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction Acta Periodica Technologica Cucurbita pepo protein isolates enyzmatic extraction pectinase cellulase |
| author_facet |
Peričin Draginja M. Radulović Ljiljana M. Trivić Svetlana Z. Dimić Etelka B. |
| author_sort |
Peričin Draginja M. |
| title |
The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction |
| title_short |
The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction |
| title_full |
The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction |
| title_fullStr |
The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction |
| title_full_unstemmed |
The use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction |
| title_sort |
use of glycohydrolase in the processing of hull-less seed variety of pumpkin in the relation to enhanced protein extraction |
| publisher |
Faculty of Technology, Novi Sad |
| series |
Acta Periodica Technologica |
| issn |
1450-7188 |
| publishDate |
2006-01-01 |
| description |
Enzyme hydrolysis optimisation of cell-wall polysaccharides of pumpkin seed with celluloses and pectinases and their mixture, in the relation to enhanced protein extraction was the objective of this study. The individual and combined effects of cell-wall-degrading enzyme activities on protein extraction combined with other process parameters: enzyme concentration and time of hydrolysis were evaluated by the Response Surface Methodology (RSM). The optimal value protein isolate yield (9 g of soluble protein from J 00 g pumpkin seed) is achieved under the following conditions: 2% enzymes (celluloses), reaction time 300 min, pH = 5.0, temperature 45°C. |
| topic |
Cucurbita pepo protein isolates enyzmatic extraction pectinase cellulase |
| url |
http://www.doiserbia.nb.rs/img/doi/1450-7188/2006/1450-71880637163P.pdf |
| work_keys_str_mv |
AT pericindraginjam theuseofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT radulovicljiljanam theuseofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT trivicsvetlanaz theuseofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT dimicetelkab theuseofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT pericindraginjam useofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT radulovicljiljanam useofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT trivicsvetlanaz useofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction AT dimicetelkab useofglycohydrolaseintheprocessingofhulllessseedvarietyofpumpkinintherelationtoenhancedproteinextraction |
| _version_ |
1725371269230100480 |