Effect of Seed Orientation and Medium Strength on <i>In vitro</i> Germination of <i>Pterocarpus marsupium</i> Roxb.

• Main Authors: Yogeshwar MISHRA, Rimi RAWAT, Brajesh NEMA, Fatima SHIRIN
• Format: Article
• Language: English
• Published: University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca 2013-12-01
• Series: Notulae Scientia Biologicae
• Online Access: http://notulaebiologicae.ro/index.php/nsb/article/view/9124

A method was developed for optimization of <i>In vitro</i> germination of an economically important timber-yielding multipurpose tree, <i>Pterocarpus marsupium</i> Roxb. The seeds inoculated in different orientation on different strengths of MS medium without any hormones showed varied response to the seed positions. The seeds inoculated on half strength medium in horizontal position recorded to produce a maximum germination (78.23%), shoot number (0.86) and root number (7.99). However, a maximum of shoot length of 3.67 cm was recorded in the quarter strength medium in the seeds inoculated in vertical down position, which was significantly higher than other media strength and positions. Our results indicate that the seed orientation including medium strength have tremendous effect on germination and seeds inoculated horizontally on half strength MS medium can be utilized to enhance <i>In vitro</i> seed germination of <i>Pterocarpus marsupium</i>.