A DNA-Based Biosensor Assay for the Kinetic Characterization of Ion-Dependent Aptamer Folding and Protein Binding

A DNA-Based Biosensor Assay for the Kinetic Characterization of Ion-Dependent Aptamer Folding and Protein Binding

Therapeutic and diagnostic nucleic acid aptamers are designed to bind tightly and specifically to their target. The combination of structural and kinetic analyses of aptamer interactions has gained increasing importance. Here, we present a fluorescence-based switchSENSE aptasensor for the detailed k...

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Bibliographic Details
Main Authors: Irene Ponzo, Friederike M. Möller, Herwin Daub, Nena Matscheko
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Molecules
Subjects:
aptamer
G-quadruplex
thrombin
folding
kinetics
switchSENSE
Online Access:https://www.mdpi.com/1420-3049/24/16/2877

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