Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i>
The outbreak of pseudorabies in China, caused by more virulent pseudorabies virus (PRV) than the classical strains, has led to considerable economic losses. In this study, PRV strain HNXY was isolated from the Henan province of China in 2015 from the pig farm with severe reproductive failure in sows...
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MDPI AG
2020-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/10/1804 |
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doaj-f415648e890946c384a1d7908f12a6fb2020-11-25T03:57:21ZengMDPI AGAnimals2076-26152020-10-01101804180410.3390/ani10101804Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i>Fengsun Wu0Yujin Lv1Shijun Zhang2Lingling Liu3Yuchen Wu4Pandeng Zhao5Zhifeng Peng6Shengli Liu7Zhonghua Zhang8Wengang Li9College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaDepartment of Animal Science, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaDepartment of Animal Science, Henan Agricultural University, Zhengzhou 450046, ChinaDepartment of Animal Science, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaThe outbreak of pseudorabies in China, caused by more virulent pseudorabies virus (PRV) than the classical strains, has led to considerable economic losses. In this study, PRV strain HNXY was isolated from the Henan province of China in 2015 from the pig farm with severe reproductive failure in sows and a high mortality in piglets. The 50% tissue culture infectious doses (TCID<sub>50</sub>) of HNXY in Vero cells were examined to be 10<sup>6.5</sup>/mL, and the neutralisation titer against Bartha-K61 was significantly higher than against HNXY when tested with the serum from Bartha-K61 vaccinated pigs. The 50% lethal doses (LD<sub>50</sub>) of HNXY to six-week-old BALB/c mice and two-month-old PRV-free pigs were both 10<sup>2.3</sup> TCID<sub>50</sub>. HNXY was classified as genotype II, and numerous amino acid variations were found in gB, gE, gC, gD, TK, and RR1 proteins, compared with PRV from other countries or those prevalent in China before 2012. The attenuated rHNXY-∆TK/∆gE was further constructed, which presented significantly smaller plaques than HNXY, as well as the similar growth kinetics. rHNXY-∆<i>TK</i>/∆<i>gE</i> was confirmed to be non-pathogenic to six-week-old BALB/c mice and zero-day-old piglets. This study isolated updated PRV promising to develop into a new vaccine candidate.https://www.mdpi.com/2076-2615/10/10/1804pseudorabies virusvirus isolationgenetic variation analysisgene-deletion strain |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Fengsun Wu Yujin Lv Shijun Zhang Lingling Liu Yuchen Wu Pandeng Zhao Zhifeng Peng Shengli Liu Zhonghua Zhang Wengang Li |
| spellingShingle |
Fengsun Wu Yujin Lv Shijun Zhang Lingling Liu Yuchen Wu Pandeng Zhao Zhifeng Peng Shengli Liu Zhonghua Zhang Wengang Li Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i> Animals pseudorabies virus virus isolation genetic variation analysis gene-deletion strain |
| author_facet |
Fengsun Wu Yujin Lv Shijun Zhang Lingling Liu Yuchen Wu Pandeng Zhao Zhifeng Peng Shengli Liu Zhonghua Zhang Wengang Li |
| author_sort |
Fengsun Wu |
| title |
Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i> |
| title_short |
Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i> |
| title_full |
Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i> |
| title_fullStr |
Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i> |
| title_full_unstemmed |
Isolation and Characterization of a Variant Psedorabies Virus HNXY and Construction of rHNXY-∆<i>TK</i>/∆<i>gE</i> |
| title_sort |
isolation and characterization of a variant psedorabies virus hnxy and construction of rhnxy-∆<i>tk</i>/∆<i>ge</i> |
| publisher |
MDPI AG |
| series |
Animals |
| issn |
2076-2615 |
| publishDate |
2020-10-01 |
| description |
The outbreak of pseudorabies in China, caused by more virulent pseudorabies virus (PRV) than the classical strains, has led to considerable economic losses. In this study, PRV strain HNXY was isolated from the Henan province of China in 2015 from the pig farm with severe reproductive failure in sows and a high mortality in piglets. The 50% tissue culture infectious doses (TCID<sub>50</sub>) of HNXY in Vero cells were examined to be 10<sup>6.5</sup>/mL, and the neutralisation titer against Bartha-K61 was significantly higher than against HNXY when tested with the serum from Bartha-K61 vaccinated pigs. The 50% lethal doses (LD<sub>50</sub>) of HNXY to six-week-old BALB/c mice and two-month-old PRV-free pigs were both 10<sup>2.3</sup> TCID<sub>50</sub>. HNXY was classified as genotype II, and numerous amino acid variations were found in gB, gE, gC, gD, TK, and RR1 proteins, compared with PRV from other countries or those prevalent in China before 2012. The attenuated rHNXY-∆TK/∆gE was further constructed, which presented significantly smaller plaques than HNXY, as well as the similar growth kinetics. rHNXY-∆<i>TK</i>/∆<i>gE</i> was confirmed to be non-pathogenic to six-week-old BALB/c mice and zero-day-old piglets. This study isolated updated PRV promising to develop into a new vaccine candidate. |
| topic |
pseudorabies virus virus isolation genetic variation analysis gene-deletion strain |
| url |
https://www.mdpi.com/2076-2615/10/10/1804 |
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