Development and validation of portable, field-deployable Ebola virus point-of-encounter diagnostic assay for wildlife surveillance

Development and validation of portable, field-deployable Ebola virus point-of-encounter diagnostic assay for wildlife surveillance

Abstract Early detection of Ebola virus spillover into wildlife is crucial for rapid response. We developed and validated a portable, cold-chain independent Ebola virus RT-qPCR assay. Methods The field syringe-based RNA extraction method was compared with a conventional laboratory-based spin-column...

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Bibliographic Details
Main Authors: Dania M. Figueroa, Eeva Kuisma, M. Jeremiah Matson, Alain U. Ondzie, Trent Bushmaker, Stephanie N. Seifert, Francine Ntoumi, Beatriz Escudero-Pérez, César Muñoz-Fontela, Chris Walzer, Sarah H. Olson, Cynthia Goma-Nkoua, Jean-Vivien Mombouli, Robert J. Fischer, Vincent J. Munster
Format: Article
Language:English
Published: BMC 2021-05-01
Series:One Health Outlook
Subjects:
Ebola virus
Portable diagnostics
Wildlife surveillance
RT-qPCR
Zoonoses
Online Access:https://doi.org/10.1186/s42522-021-00041-y

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