In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid

In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid

Thunbergia laurifolia Lindl. (Thunbergiaceae family) was shown to be a natural antioxidant source to cope with oxidative stress. By DPPH and FRAP antioxidant assays, this study found that rosmarinic acid exhibited the highest antioxidant activity followed by leaf and stem ethanolic extracts of T....

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Main Authors: Parichart Hongsing, Chanida Palanuvej, Nijsiri Ruangrungsi
Format: Article
Language:English
Published: Prince of Songkla University 2020-06-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
thunbergia laurifolia
rosmarinic acid
reactive oxygen species
antioxidant activity
Online Access:https://rdo.psu.ac.th/sjstweb/journal/42-3/17.pdf
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spelling doaj-f403314544c146cbae42b15eb49bd5452020-11-25T03:45:21ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952020-06-0142361562010.14456/sjst-psu.2020.78In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acidParichart Hongsing0Chanida Palanuvej1Nijsiri Ruangrungsi2Public Health Sciences Program, College of Public Health Sciences, Chulalongkorn University, Pathum Wan, Bangkok, 10330 ThailandPublic Health Sciences Program, College of Public Health Sciences, Chulalongkorn University, Pathum Wan, Bangkok, 10330 ThailandPublic Health Sciences Program, College of Public Health Sciences, Chulalongkorn University, Pathum Wan, Bangkok, 10330 ThailandThunbergia laurifolia Lindl. (Thunbergiaceae family) was shown to be a natural antioxidant source to cope with oxidative stress. By DPPH and FRAP antioxidant assays, this study found that rosmarinic acid exhibited the highest antioxidant activity followed by leaf and stem ethanolic extracts of T. laurifolia. By intracellular anti-oxidation assay against H2O2-induced ROS in EA.hy926 endothelial cells using 2′,7′-dichlorodihydrofluorescein diacetate as a probe, all tested samples exhibited marginal protection, i.e. T. laurifolia leaf extract (77.31±0.66%), rosmarinic acid (88.10±6.105%), and T. laurifolia stem extract (91.30±0.86%), compared to the control (100%). Cytotoxicity against five cancer and two normal cell lines were evaluated by MTT cell viability assay. Rosmarinic acid and the leaf extract inhibited cell viability selectively in each cell line, whereas the stem extract revealed no cytotoxicity (IC50≥100 µg/mL) in any of the cell lines https://rdo.psu.ac.th/sjstweb/journal/42-3/17.pdfthunbergia laurifoliarosmarinic acidreactive oxygen speciesantioxidant activity
collection DOAJ
language English
format Article
sources DOAJ
author Parichart Hongsing
Chanida Palanuvej
Nijsiri Ruangrungsi
spellingShingle Parichart Hongsing
Chanida Palanuvej
Nijsiri Ruangrungsi
In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid
Songklanakarin Journal of Science and Technology (SJST)
thunbergia laurifolia
rosmarinic acid
reactive oxygen species
antioxidant activity
author_facet Parichart Hongsing
Chanida Palanuvej
Nijsiri Ruangrungsi
author_sort Parichart Hongsing
title In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid
title_short In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid
title_full In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid
title_fullStr In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid
title_full_unstemmed In vitro biological activities of Thunbergia laurifolia stem and leaf with reference to rosmarinic acid
title_sort in vitro biological activities of thunbergia laurifolia stem and leaf with reference to rosmarinic acid
publisher Prince of Songkla University
series Songklanakarin Journal of Science and Technology (SJST)
issn 0125-3395
publishDate 2020-06-01
description Thunbergia laurifolia Lindl. (Thunbergiaceae family) was shown to be a natural antioxidant source to cope with oxidative stress. By DPPH and FRAP antioxidant assays, this study found that rosmarinic acid exhibited the highest antioxidant activity followed by leaf and stem ethanolic extracts of T. laurifolia. By intracellular anti-oxidation assay against H2O2-induced ROS in EA.hy926 endothelial cells using 2′,7′-dichlorodihydrofluorescein diacetate as a probe, all tested samples exhibited marginal protection, i.e. T. laurifolia leaf extract (77.31±0.66%), rosmarinic acid (88.10±6.105%), and T. laurifolia stem extract (91.30±0.86%), compared to the control (100%). Cytotoxicity against five cancer and two normal cell lines were evaluated by MTT cell viability assay. Rosmarinic acid and the leaf extract inhibited cell viability selectively in each cell line, whereas the stem extract revealed no cytotoxicity (IC50≥100 µg/mL) in any of the cell lines
topic thunbergia laurifolia
rosmarinic acid
reactive oxygen species
antioxidant activity
url https://rdo.psu.ac.th/sjstweb/journal/42-3/17.pdf
work_keys_str_mv AT paricharthongsing invitrobiologicalactivitiesofthunbergialaurifoliastemandleafwithreferencetorosmarinicacid
AT chanidapalanuvej invitrobiologicalactivitiesofthunbergialaurifoliastemandleafwithreferencetorosmarinicacid
AT nijsiriruangrungsi invitrobiologicalactivitiesofthunbergialaurifoliastemandleafwithreferencetorosmarinicacid
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