Evaluating the cleavage efficacy of CRISPR-Cas9 sgRNAs targeting ineffective regions of Arabidopsis thaliana genome

Evaluating the cleavage efficacy of CRISPR-Cas9 sgRNAs targeting ineffective regions of Arabidopsis thaliana genome

The CRISPR-Cas9 system has recently evolved as a powerful mutagenic tool for targeted genome editing. The impeccable functioning of the system depends on the optimal design of single guide RNAs (sgRNAs) that mainly involves sgRNA specificity and on-target cleavage efficacy. Several research groups h...

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Bibliographic Details
Main Authors: Afsheen Malik, Alvina Gul, Faiza Munir, Rabia Amir, Hadi Alipour, Mustafeez Mujtaba Babar, Syeda Marriam Bakhtiar, Rehan Zafar Paracha, Zoya Khalid, Muhammad Qasim Hayat
Format: Article
Language:English
Published: PeerJ Inc. 2021-05-01
Series:PeerJ
Subjects:
CRISPR-Cas9
Cleavage efficacy
Non-coding region
sgRNA design tool
Ineffective region
Genome editing
Online Access:https://peerj.com/articles/11409.pdf

Internet

https://peerj.com/articles/11409.pdf

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