Mitochondrial Polymorphisms, in The D-Loop Area, Are Associated with Brain Tumors

• Main Authors: Donya Altafi, Soha Sadeghi, Hamed Hojatian, Maryam Torabi Afra, Safoura Pakizeh Kar, Mojtaba Gorji, Massoud Houshmand
• Format: Article
• Language: English
• Published: Royan Institute (ACECR), Tehran 2019-06-01
• Series: Cell Journal
• Subjects: Brain Tumor; D-Loop; Mitochondrial DNA
• Online Access: https://celljournal.org/journal/article/abstract/5947
• ISSN: 2228-5806; 2228-5814

Objective: This study was carried out to evaluate the relationship between mtDNA D-loop variations and the pathogenesis of a brain tumor. Materials and Methods: In this experimental study, 25 specimens of brain tumor tissue with their adjacent tissues from patients and 454 blood samples from different ethnic groups of the Iranian population, as the control group, were analysed by the polymerase chain reaction (PCR)-sequencing method. Results: Thirty-six variations of the D-loop area were observed in brain tumor tissues as well as the adjacent normal tissues. A significant difference of A750G (P=0.046), T15936C (P=0.013), C15884G (P=0.013), C16069T (P=0.049), T16126C (P=0.006), C16186T (P=0.022), T16189C (P=0.041), C16193T (P=0.045), C16223T (P=0.001), T16224C (P=0.013), C16234T (P=0.013), G16274A (P=0.009), T16311C (P=0.038), C16327T (P=0.045), C16355T (P=0.003), T16362C (P=0.006), G16384A (P=0.042), G16392A (P=0.013), G16394A (P=0.013), and G16477A (P=0.013) variants was found between the patients and the controls. Conclusion: The results indicated individuals with C16069T [odds ratio (OR): 2.048], T16126C (OR: 2.226), C16186T (OR: 3.586), G16274A (OR: 4.831), C16355T (OR: 7.322), and T16362C (OR: 6.682) variants with an OR more than one are probably associated with a brain tumor. However, given the multifactorial nature of cancer, more investigation needs to be done to confirm this association.