Environment-insensitive two-photon ratiometric probe for in cellulo quantitative measurement of hydrogen peroxide

• Main Authors: Seo Won Cho, Yong Woong Jun, Ye Jin Reo, Sourav Sarkar, Kyo Han Ahn
• Format: Article
• Language: English
• Published: Elsevier 2021-01-01
• Series: Results in Chemistry
• Subjects: Ratiometric imaging; Quantitative analysis; Environment-insensitive probe; Hydrogen peroxide; Two-photon imaging
• Online Access: http://www.sciencedirect.com/science/article/pii/S2211715621000229

The quantitative analysis of a biological analyte directly through fluorescence imaging is essential in biomedical sciences but remains as a challenge owing to the environment-sensitive nature of fluorescence intensity. We show that a fluorescent hydrogen peroxide probe based on an in cellulo super-bright benzothiazolyl-benzocoumarin dye of which emission properties are insensitive to environmental changes offers a reliable and practical means for the quantitative analysis of hydrogen peroxide in cells and tissues directly through ratiometric imaging both under one-photon and two-photon excitation conditions. Different cellular hydrogen peroxide concentrations were determined for several cell lines and also for tumour and normal tissues.