Biodentine^™ Boosts, WhiteProRoot^®MTA Increases and Life^® Suppresses Odontoblast Activity

• Main Authors: Anabela Paula, Mafalda Laranjo, Carlos Miguel Marto, Ana Margarida Abrantes, João Casalta-Lopes, Ana Cristina Gonçalves, Ana Bela Sarmento-Ribeiro, Manuel M. Ferreira, Maria Filomena Botelho, Eunice Carrilho
• Format: Article
• Language: English
• Published: MDPI AG 2019-04-01
• Series: Materials
• Subjects: biocompatibility; biomaterials; cytotoxicity; dentinogenesis; pulp capping; odontoblast
• Online Access: https://www.mdpi.com/1996-1944/12/7/1184

(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life^®), mineral trioxide aggregate (WhiteProRoot^®MTA) and calcium silicate (Biodentine^™), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life^® induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot^®MTA and Biodentine™ induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine™. (4) Conclusions: The results of WhiteProRoot^®MTA confirm its indication for these therapies, justifying its recognition as the “gold standard„. Biodentine™ may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does.