| Summary: | Large quantities of oyster mushroom (Pleurotus sajor caju. )spent medium (MSM) were disposed of as agricultural waste to the environment and thereby constituting environmental pollution. Therefore the study was conducted to examine the feasibility of using MSM as glucose source in comparison to RSD and GM under different NaOH pretreatment techniques and at the same enzymatic saccharification condition. The composition analysis proved that decreases in lignocellulosic contents occurred after cultivation of The amount of lignin, hemicelluloses, and cellulose in MSM showed lower values than those in RSD and GM which were 22.40, 27.93, and 27.97% respectively. The surface morphology of MSM appeared to be rough and broken and traces of hyphen on the fibre surface were observed as a result from the mushroom cultivation process. RSD and FM had an even and smooth flat surface, indicating a rigid and highly ordered surface structure. The highest glucose yield were obtained from MSM under all three NaOH pretreatment techniques (autoclaving at 121°C, heating in water bath, soaking at room temperature), that were 31.07, 30.13, and 21.97, g/ dry substrate respectively. The amount of glucose obtained from RSD and GM under the same treatments were comparable.
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